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用ITS序列确定小麦B基因组的可能供体间的关系

张文驹,瞿礼嘉,高巍,顾红雅,陈章良,陈家宽   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-09-10 发布日期:1999-09-10
  • 通讯作者: 张文驹

The phylogenetic relationships among the possible donors of B-genome of common wheat based on internal transcribed spacer (ITS) sequences

ZHANG Wen-Ju, QU Li-Jia, GAO Wei, GU Hong-Ya, CHEN Zhang-Liang, CHEN Jia-Kuan   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-09-10 Published:1999-09-10
  • Contact: ZHANG Wen-Ju

摘要:

对小麦B基因组的可能供体山羊草属Aegilops sect.Sitopsis的5个种的核糖体DNA的内部转
录区(ITS)进行了PCR扩增和克隆,井测定ITSl和ITS2的序列,用ITSl+ITS2的序列重建了
Aegilops sect.Sitopsis中5个种的系统发育关系。结果表明,斯卑尔脱山羊草Ae.speltoides是sect.
Sitopsis中特殊的一个种,它与该组其余4种间的平均遗传距离是后者彼此间平均遗传距离的3倍,Ae.
speltoides与同组其余4个种的分离要比后者相互间的分离早得多;在拟斯卑尔脱组Sect.Sitopsis的5
个种中,长柱山羊草Ae.longissima与沙融山羊草Ae.sharonensis的关系最近。ITS序列可以进一步
用来作为确定B基因组起源的分子标记。

关键词: 小麦, 山羊草属拟斯卑尔脱组, ITS序列, 系统发育, B基因组

Abstract:

The ITS regions of 5 species in Aegilops sect. Sitopsis, the possible donors of B-
genome of common wheat, were amplified by PCR, cloned and sequenced. The phylogenetic
relationships among 5 species in Aegilops sect. Sitopsis were constructed based on ITS1 +
ITS2 sequences. The results demonstrated that Ae. speltoides was a distinct species in
Aegilops sect. Sitopsis. The average of the pairwise distances between Ae. speltoides and
the other four species was three times as high as that among the latter four. Ae. speltoides
was the earliest lineage of the section under question. Relationship between Ae. longissima
and Ae. sharonensis was the closest in Aegilops sect. Sitopsis. Sequence of ITS region
could be used as a molecular marker to identify origin of B-genome in polyploid wheats.

Key words: Wheat, Aegilops sect.Sitopsis, ITS sequence, Phylogeny, B-genome