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芍药属牡丹组的系统学研究——基于RAPD分析

邹喻苹,蔡美琳,王子平   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-05-10 发布日期:1999-05-10
  • 通讯作者: 邹喻苹

Systematic studies on Paeonia sect. Moutan DC. based on RAPD analysis

ZOU Yu-Ping, CAI Mei-Lin, WANG Zi-Ping   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-05-10 Published:1999-05-10
  • Contact: ZOU Yu-Ping

摘要:

芍药属牡丹组(Paeonia sect.Moutan DC)是落叶亚灌木,其野生类群为我国特有。长期以来不同
学者根据形态性状对这个组中种的分类处理不断修正,不断有新种描述。我们采用RAPD标记分析了
牡丹组种内与种间遗传关系。从10个RAPD引物获得121个多态位点。用UPGMA方法构建的树系
图表明每个种的所有个体都各自聚为一支,种内的相似性系数为0.60~0.90,因此现有的7个种能很好
地区分开来。P.delavayi与P.ludlowii相似性系数为0.60,聚为一支;P.jishanensis与P.rockii、P.
ostii、P.qiui以及P.decomposita之间的相似性系数为0.48,聚为一大支。这两支与肉质花盘亚组和革
质花盘亚组相对应。这些结果与洪德元根据形态性状对该组所做的分类处理基本相符。我们认为
RAPD技术用于牡丹基因组分析是灵敏而行之有效的工具。

关键词: 随机扩增的多态性DNA, 芍药属牡丹组, 系统学

Abstract:

 Plants in Paeonia sect. Moutan DC., whose wild types are endemic to China, are
 deciduous subshrubs. Taxonomic treatments of most species in this section have long been in
 dispute. To address this question, both intraspecific and interspecific relationships of the
 species in this section were analyzed using RAPD markers. The dendrogram constructed by
 UPGMA showed that the accessions of the same species were always grouped together earlier
 than those of different species. The intraspecific similarity coefficients ranged from 0.60 to
 0.90, grouping precisely those species of the same subsection together. Hence, the seven
 species under question can be well distinguished from each other. The similarity coefficient
 between P. delavayi and P. ludlowii was 0.60, and they were clustered in a clade. The
 similarity coefficients between P. jishanensis and the three species P. rockii, P. ostii, P.
 qiui, and between P. jishanensis and P. decomposita were both 0.48. These five species
 were clustered in another clade. These two clades corresponded well to Subsect. Delavayanae
 and Subsect. Vaginatae. Our results support the taxonomic treatment of Sect. Moutan re-
 cently proposed by Hong (1998,1997).

Key words: RAPD, Paeonia sect. Moutan DC, Systematics