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中国科学院大学学报 ›› 2012, Vol. 29 ›› Issue (1): 38-46.DOI: 10.7523/j.issn.2095-6134.2012.1.006

• 环境科学与地理学 • 上一篇    下一篇

短程硝化-ANAMMOX反应器处理城市污水过程中脱氮微生物的群落解析

王惠1, 易鹏2, 张树军2, 刘研萍3, 陶莹4, 周军2, 刘新春1   

  1. 1. 中国科学院研究生院资源与环境学院, 北京 100049;
    2. 北京城市排水集团有限责任公司, 北京 100022;
    3. 北京化工大学环境科学与工程系, 北京 100029;
    4. 河南源通环保工程有限公司, 河南开封 475000
  • 收稿日期:2011-04-12 修回日期:2011-04-20 发布日期:2012-01-15
  • 基金资助:

    国家科技支撑计划(2007BAD87B12)和中国科学院知识创新工程重要方向项目基金(KSCX2-YW-G-054)资助

Functional microbial community composition analysis in the laboratory-scale stable partial nitrifying-ANAMMOX municipal wastewater reactor

WANG Hui1, YI Peng2, ZHANG Shu-Jun2, LIU Yan-Ping3, TAO Ying4, ZHOU Jun2, LIU Xin-Chun1   

  1. 1. College of Resources and Environment, Graduate University of Chinese Academy of Sciences, Beijing 100049, China;
    2. Beijing Drainage Group Co Ltd, Beijing 100022, China;
    3. Department of Environmental Sciences and Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
    4. Yuan Tong Environmental Engineering Co Ltd, Kaifeng 475000, Henan, China
  • Received:2011-04-12 Revised:2011-04-20 Published:2012-01-15

摘要:

对基于短程硝化-厌氧氨氧化反应体系实现低氨氮城市生活污水的稳定脱氮进行了实验分析. 稳定时,反应体系的总氮去除率达80%. 对反应器活性污泥中脱氮微生物进行了多样性分析. 结果表明,低溶氧的短程硝化反应器中,与Nitrosomonas europaea/Nitrosococcus mobilis相似性较高的氨氧化细菌,以及分别与Nitrobacter vulgaris Nitrospira defluvii相似性较高的亚硝酸盐氧化菌是优势菌种;厌氧氨氧化反应器中,与Kuenenia stuttgartiensis相似性较高的厌氧氨氧化细菌是优势菌种. 并对厌氧氨氧化反应器中总细菌的群落结构进行了解析,发现大量属于Proteobacteria门、Chloroflexi门和Bacteroidetes/Chlorobi门的细菌与anammox细菌共存.

关键词: 短程硝化, 厌氧氨氧化, 氨氧化细菌, 亚硝酸盐氧化菌

Abstract:

A partial nitrification-ANAMMOX reactor system for domestic wastewater was set up, and the composition of N-related functional bacteria in the activated sludge were studied. During the stable period, the TN removal rate reached to about 80%. In the partial nitrification system, the dominant AOB is Nitrosomonas europaea/Nitrosococcus mobilis cluster-like bacteria, and the dominant NOB are Nitrobacter vulgaris-like bacteria and Nitrospira defluvii-like bacteria. In the anammox reactor, Kuenenia stuttgartiensis-like bacteria is the dominant anammox bacteria. Numerous members of proteobacteria, Chloroflexi, and Bacteroidetes/Chlorobi phylum are found to co-exist with anammox bacteria.

Key words: partial nitrification, anammox, ammonium oxidation bacteria (AOB), nitrite oxidation bacteria (NOB)

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