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中国科学院大学学报 ›› 2014, Vol. 31 ›› Issue (2): 182-186.DOI: 10.7523/jssn.2095-6134.2014.02.007

• 化学与生命科学 • 上一篇    下一篇

基于MSRF的黄海漂浮浒苔与定生浒苔的甲基化差异分析

王萌1,2, 崔玉琳1, 王寅初1,2, 刘正一3, 秦松1   

  1. 1. 中国科学院烟台海岸带研究所, 山东 烟台 264003;
    2. 中国科学院大学, 北京 100049;
    3. 南京农业大学资源环境学院, 南京 210095
  • 收稿日期:2013-05-06 修回日期:2013-05-23 发布日期:2014-03-15
  • 通讯作者: 秦松,E-mail:sqin@yic.ac.cn
  • 基金资助:

    国家自然科学基金(41176144);海洋公益性行业科研专项(20120527)和山东省自然科学基金(JQ200914)资助

MSRF analyses of methylation differences between two Enteromorpha prolifera populations (attached and free-floating) in Yellow Sea

WANG Meng1,2, CUI Yulin1, WANG Yinchu1,2, LIU Zhengyi3, QIN Song1   

  1. 1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2013-05-06 Revised:2013-05-23 Published:2014-03-15

摘要:

应用甲基化敏感限制性指纹技术(MSRF),利用对DNA甲基化敏感与不敏感的限制性内切酶组合产生基因组的酶切多态性差异图谱. 发现漂浮浒苔可能在BstUI位点发生了甲基化增强的现象,推测表观遗传水平上的改变可能推动了漂浮浒苔对黄海自由漂浮生活的快速适应.

关键词: 浒苔, 表观遗传学, 甲基化, 甲基化敏感的限制性指纹技术

Abstract:

The free-floating population of Enteromorpha prolifera, which results in the blooms in Yellow Sea, has remarkable phenotypic variations compared to the attached population. We applied methylation-sensitive restriction fingerprinting (MSRF) to E. prolifera for methylation polymorphism with methylation-sensitive enzyme digestion. Our results showed that the free-floating sample might be abnormally hypermethylated at BstUI sites. It is suggested that these changes at epigenetic level may promote the rapid adaptation of E. prolifera to free-floating lives in Yellow Sea.

Key words: Enteromorpha prolifera, epigenetics, methylation, methylation-sensitive restriction fingerprinting

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