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中国科学院大学学报 ›› 2004, Vol. 21 ›› Issue (4): 488-493.DOI: 10.7523/j.issn.2095-6134.2004.4.010

• 论文 • 上一篇    下一篇

酶法合成13C标记尿苷二磷酸葡萄糖的核磁鉴定

祁超1,2, 刘立岩2   

  1. 1. 中国科学院长春应用化学研究所稀土化学与物理重点实验室, 合肥 230027;
    2. 中国科学院长春应用化学研究所稀土化学与物理重点实验室, 长春 130022
  • 发布日期:2004-07-10
  • 通讯作者: 祁超,Email:qichao@ustc.edu.Cn.
  • 基金资助:

    国家自然科学基金(29701005);中国科学院基础局重大项目(KJ951A150402)资助

Characterization of13C2Labeled Uridine Diphosphoglucoseof Enzymatic Synthesis by NMR

QI Chao1,2, LIU Li-Yan2   

  1. 1. Key Laboratory of Structural Biology, School of Life Science, University of Science and Technology of China, Hefei 230027, China;
    2. Key Laboratory of Chemistry and Physics of Rare Earth, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Published:2004-07-10

摘要:

建立了一种经济、简便的一步酶法,大量合成尿苷二磷酸葡萄糖.在酶法合成中,用UTP代替ATP,并建立了UTP的再生系统 ;建立了反复补加法和酶回收法,使酶的利用率达到最高.UDP-[4-13C]-葡萄糖首次用该法在 0 5g规模上合成.总产率为 69 7%.UDP-[4-13C]-葡萄糖的核磁共振 (NMR)结果表明,文献报道的UDPG的1H和13 C核磁共振谱中葡萄糖 4位C的归属是错误的,从而纠正了文献中关于UDPG核磁共振谱归属中的部分错位.

关键词: 尿苷二磷酸葡萄糖, 酶法合成, 一步酶法, 核磁共振

Abstract:

The authors set up enzymatic method of one step to synthesize UDPG in much higher yields, which iseconomical and cheap. In enzymatic synthesis, UTP is used to replace ATP, and repetitive system of UTP is setup. Repetitive addition and recovery of enzyme are used to take advantage of enzyme. UDP-[4-13C]-glucose are firstsynthesized on 0.5 g scale by this method. The overall yield is 69.7 %. NMR spectra of 13C-labelled UDPGmakecertain the assignment of C-4 of the glucose residue and suggest that part of the results in the literatures about theassignment of UDPG are wrong

Key words: UDPG, enzymatic synthesis, enzymatic method of one step, NMR

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