关键词: 细胞膜电穿孔, 强脉冲电场, 生物效应

Abstract:

In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine Bisothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects.

Key words: electroporation of cell membrane, high intense pulsed electric field, biological effect