基于热裂解气相色谱质谱联用技术的贝叶经植物种属鉴别*

doi:10.7523/j.ucas.2024.078

中国科学院大学学报

基于热裂解气相色谱质谱联用技术的贝叶经植物种属鉴别^*

陈清乐^1,2, 杨益民², 韩宾², 蒋洪恩^2†

1 中国科学院古脊椎动物与古人类研究所,中国科学院脊椎动物演化与人类起源重点实验室北京 100044;
2 中国科学院大学人文学院考古学与人类学系北京 100049

收稿日期:2024-08-08 修回日期:2024-11-28 发布日期:2024-12-23
通讯作者: ^†E-mail:jianghongen@ucas.ac.cn
基金资助:
^*国家重点研发计划重点项目（2022YFF0903901）资助

Species identification of palm leaf manuscript based on Py-GC/MS

CHEN Qingle^1,2, YANG Yimin², Han Bin², JIANG Hongen²

1 Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China;
2 Department of Archaeology and Anthropology, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2024-08-08 Revised:2024-11-28 Published:2024-12-23

摘要/Abstract

摘要： 贝叶经制作材料主要来自贝叶棕（Corypha umbraculifera）和糖棕（Borassus flabellifer）两种棕榈科植物叶片。采用微损、快速的方法判断植物来源,有利于深入认知贝叶经的利用,为文物保护工作提供参考。本研究通过模拟实验制得一批标准样品,寻找区分两种棕榈的标准,并在已知植物来源的贝叶经工艺品上进行验证。利用热裂解气相色谱质谱分析（Py-GC/MS）,结合多元统计分析方法区分贝叶棕和糖棕。结果发现已知样品存在多种三萜类物质,其中环桉烯醇衍生物仅在贝叶棕中出现;两种羽扇豆烷型三萜和环鸦片甾烯醇及何帕烷型三萜仅于糖棕中出现。羽扇豆烷型三萜化合物和何帕烷型三萜在糖棕中稳定存在。对10种共有的木质素单体相对含量进行主成分分析,结果显示贝叶棕组和糖棕组具有良好的区分效果。结合特征化合物和主成分分析方法成功鉴别出多种类型的现代贝叶经工艺品植物来源,为贝叶经文物植物种属的快速和微量鉴别研究提供新思路。

关键词: 贝叶经, Py-GC/MS, 种属鉴别

Abstract: The materials for making palm leaf manuscripts mainly come from the leaves of two palms, Corypha umbraculifera and Borassus flabellifer. Applying a micro-destructive and rapid method to determine the plant species origin is conducive to in-depth cognition of the utilization of the palm leaf manuscripts and provides a reference for its future conservation. In this study, a batch of standard samples were produced through simulation experiments to search the criteria to distinguish the two palm species and validate them on the artifacts of the palm leaf manuscripts of known plant species. Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) was utilized in combination with multivariate statistical analysis to differentiate the C. umbraculifera and B. flabellifer. The results revealed the presence of several triterpenoids in the samples, among which cycloeucalenol derivatives were found only in C. umbraculifera; two lupane-type triterpenoids and one cyclolaudenol and hopane-type triterpenoids were found only in B. flabellifer. The lupane-type triterpene and hopane-type compounds are stable in B. flabellifer. The relative contents of 10 lignin monomers were selected for principal component analysis (PCA), and the results showed a good differentiation between the C. umbraculifera group and the B. flabellifer group. The combination of the characteristic compounds and the PCA method successfully identified different types of plant sources of modern palm leaf manuscripts, which provides a new idea for the rapid and trace identification study of the species and genera of ancient palm leaf manuscripts.

Key words: palm leaf manuscript, Py-GC/MS, species identification

中图分类号:

陈清乐, 杨益民, 韩宾, 蒋洪恩. 基于热裂解气相色谱质谱联用技术的贝叶经植物种属鉴别^*[J]. 中国科学院大学学报, DOI: 10.7523/j.ucas.2024.078.

CHEN Qingle, YANG Yimin, Han Bin, JIANG Hongen. Species identification of palm leaf manuscript based on Py-GC/MS[J]. Journal of University of Chinese Academy of Sciences, DOI: 10.7523/j.ucas.2024.078.

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基于热裂解气相色谱质谱联用技术的贝叶经植物种属鉴别^*

Species identification of palm leaf manuscript based on Py-GC/MS

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