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胡桃属十种植物的过氧化物同工酶分析

杨自湘, 奚声珂   

  1.  (中国林业科学研究院林业研究所,北京)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1989-01-10 发布日期:1989-01-10
  • 通讯作者: 杨自湘

A Study on Isozymes of Peroxidase of 10 Species in Juglans L.

Yang Zi-Xiang, Xi Sheng-Ke   

  1.  (Institute of Forestry, Chinese Academy of Forestry, Beijing)
  • Received:1900-01-01 Revised:1900-01-01 Online:1989-01-10 Published:1989-01-10
  • Contact: Yang Zi-Xiang

摘要:

用丙烯酰胺凝胶电泳分离了胡桃属Juglans L.10种植物80份材料的过氧化物同工
  酶,共出现16种酶谱。  种内酶谱多态,但酶带间的差异小于种间酶带差异。用“酶谱距离”
  及特异酶带可将10个种分成4组,与胡桃属分类基本吻合。  黑核桃组的酶带最多。胡桃楸
  次之,核桃及灰核桃最少。根据同工酶的产生与进化的关系,可认为黑核桃与胡桃楸比核桃、
  灰核桃进化。胡桃属内平均“酶谱距离”为0.52,推测该属应发生在晚白垩纪到古新世。

Abstract:

 Isozymes of peroxidase of 10 species in Juglans L. were analyzed by using poly-
crylamide gel electrophoresis, and, as a result, 16 different patterns of isozymes were observed.
Polymophism of isozymes patterns appears within species and more significant differences in pat-
tern between species have been found. “Zymogram distance” was measured for each species
pair and section pair.  The ten species may be divided into 4 groups according to their “zymo-
gram distance” and specific bands, which accords with the classical taxonomy of Juglans L..
Evolutionary relationship among species and rate of evolution for Juglans L. are discussed.

Key words: Juglans, Lsozyme