实时BIA技术对蛋白A与免疫球蛋白G相互作用的研究

中国科学院大学学报 ›› 2000, Vol. 17 ›› Issue (2): 34-38.

实时BIA技术对蛋白A与免疫球蛋白G相互作用的研究

裴仁军, 杨秀荣, 汪尔康

中国科学院长春应用化学研究所电分析化学开放研究实验室, 国家电化学和光谱研究中心长春 130022

收稿日期:2000-11-15 发布日期:2000-03-15
作者简介:裴仁军,男,1971年生,博士后
基金资助:
国家自然科学基金资助项目!(200075027)

Studies on Interaction between Protein A and Immunoglobul in G Using Real-Time BIA Technique

Pei Renjun, Yang Xiurong, Wang Erkang

Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Received:2000-11-15 Published:2000-03-15

摘要/Abstract

摘要：

应用实时BIA技术,探讨了蛋白A与小鼠免疫球蛋白G (MIgG)之间的相互作用,求出相互作用的动力学速率常数ka=5.07×10⁴ (mol/L)^-1s^-1,kd=9.65×10^-5(s^-1),结合常数KA=5.25×10⁸(mol/L)^-1.同时使用蛋白A固定的传感片用于MIgG浓度的检测,在 0.64～ 12mg/L浓度区间内,MIgG的响应值与其浓度有非常好的线性关系.

关键词: 实时BIA技术, 蛋白A, 免疫球蛋白G, 动力学常数

Abstract:

The interaction betw een protein A and MIgG has been investigated.Some interesting kinetic parameters have been obtained.The kinetic constants between protein A and MIgG that we have obtained f rom the BIA senso rg ram is as follow ing :associate rate constant :ka=5.07×10⁴ (mol/L)^-1s^-1, dissociate rate constant :kd=9.65×10^-5(s^-1), af finity constant :KA=5.25×10⁸(mol/L)^-1.The concentrat ion detection of MIgG, using protein A immobilized sensor chip, has been also studied.A good linear relationship betw een SPR response unit s and MIgG concent ration has been found in the range of 0.64 ～ 12mg/ L.

Key words: Real-time BIA technique, protein A, immunoglobulin G, kinetic constants

中图分类号:

Q-33

裴仁军, 杨秀荣, 汪尔康. 实时BIA技术对蛋白A与免疫球蛋白G相互作用的研究[J]. 中国科学院大学学报, 2000, 17(2): 34-38.

Pei Renjun, Yang Xiurong, Wang Erkang. Studies on Interaction between Protein A and Immunoglobul in G Using Real-Time BIA Technique[J]. , 2000, 17(2): 34-38.

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