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裸子植物的生化系统学(四)——杉科植物的种子蛋白和针叶过氧化物酶

胡志昂, 王洪新, 刘长江   

  1. (中国科学院植物研究所,北京)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-12-18 发布日期:1986-12-18
  • 通讯作者: 胡志昂

Biochemical Systematics of Gymnosperms (4)—Seed Protein Peptides and Needle Peroxidases of Taxodiaceae

Hu Zhi-Ang, Wang Hong-Xin, Liu Chang-Jiang   

  1.  (Institute of Botany, Academia Sinica, Beijing)
  • Received:1900-01-01 Revised:1900-01-01 Online:1986-12-18 Published:1986-12-18
  • Contact: Hu Zhi-Ang

摘要:

用SDS聚丙烯酰胺凝胶电泳分析了杉科全部10属共13种植物的种子蛋白多肽。它们
    都有分子量为32、24和10K(千道尔顿)的多肽。每个分类群一般只有2至4个主要多肽,构
    成种子蛋白总含量的一半;而且其分子量都分布在24—26K及29—36K两个区域内。  因此,
    杉科植物种子蛋白谱与红豆杉科、三尖杉科相似,而不同于松科。属内不同种之间,种子蛋白
    组成和针叶过氧化物酶电泳谱都没有看到显著差别。根据这两种蛋白质资料,表明杉科各属
除红杉、巨杉、落羽杉相互很接近外,其他各属间关系都比较远。但是还不能说杉科可以分立几个科。

关键词: 杉科, 种子蛋白, 过氧化物酶

Abstract:

An analysis of seed protein peptides by using SDS polyacrylamide gel electropho-
resis, indicates that all members in Taxodiaxeae share three peptides with molecular weights of
24, 32 and 10 kilodaltons. Generally, seeds of this family contain 2 to 4 major peptides, and
their molecular weights are distributed in two regions: 24-26 K and 29-36 K respectively. The
quantity of these major peptides is near or even more than 50% of seed total protein content.
This kind of protein profiles is similar to that of Taxaceae and Cephalotaxaceae rather than to
that of Pinaceae.  On the other hand, a considerable difference was observed in peptides with
higher molecular weights. Among different species within a genus, no difference has been found
both in their seed protein profiles and in their needle peroxidases.  Based on the data cited here,
it seems that the relationships among most genera of Taxodiaceae are not so closed as the rela-
tionship among following genera: Sequoia, Sequoiadendron and Taxodium.

Key words: Taxodiaceae, Seed Protein, Peroxidase