rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用

中国科学院大学学报

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rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用

汪小全,李振宇

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-03-10 发布日期:1998-03-10
通讯作者: 汪小全

The Application of Sequence Analysis of rDNA Fragment to the Systematic Study of the Subfamily Cyrtandroideae (Gesneriaceae)

WANG Xiao-Quan, LI Zhen-Yu

Received:1900-01-01 Revised:1900-01-01 Online:1998-03-10 Published:1998-03-10
Contact: WANG Xiao-Quan

摘要/Abstract

摘要：

本文测定了苦苣苔亚科4族、5属、5种植物的核糖体DNA中的内转录间隔区(ITS)序列及5．8s

rRNA基因的3′端序列。这几种苦苣苔亚科植物的ITS-1的长度范围为234～258 bp，ITS-2的长度范

围为218～246bp。Whytockia bijieensis的ITS-1(258bP)和ITS-2(218 bp)在长度、序列及GC含量上

均与其它几个种有较大差异，其代表的尖舌苣苔族可能很早就自苦苣苔亚科的祖先沿单独的一个分支

演化。以w．bijieensis作为功能性外类群，运用PAUP软件分析仅得到一个最简约树。在简约树上，

Cyrtandra umbelliferm、Briggsia longipes和Anna mollifolia形成一个单系群，bootstrap分析对该分支的

支持强度达97％，Chirita crasslfolia位于该分支的基部。由于系统树上Cyrtandra umbellifera代表的

浆果苣苔族和Anna mollifolid代表的芒毛苣苔族均起源于长蒴苣苔族，结合这3个族在形态上存在过

渡系列，建议将浆果苣苔族和芒毛苣苔族均并入长蒴苣苔族。

关键词: 苦苣苔亚科, ITS, 分子系统发育

Abstract:

A molecular phylogeny of the subfamily Cyrtandroideae represented by five genera

of four tribes was constructed using sequence analysis of the internal transcribed spacers

(ITS) and partial 5.8 S rRNA gene (3’end) of nuclear ribosomal DNA. Direct PCR

sequencing method was used in the study. The sequences of ITS-1 in the five species range

from 234 bp to 258 bp in size and those of ITS-2 from 218 bp to 246 bp. The ITS-1 (258

bp) and ITS-2 (218 bp) of Whytockia bijieensis differ greatly from those of the other species

in size, sequence and G + C content, and therefore the tribe Klugieae represented by W.

bijieensis may have diverged from the ancestor of the subfamily Cyrtandroideae at a very

early time. In PAUP analysis, W. bijieensis was used as the functional outgroup, and only

one most parsimonious Fitch tree was obtained through exhaustive search. The tree has 353

steps, with CI = 0. 932 and RI = 0. 529. In the tree, Chirita crassifolia is basal to a

monophyletic group comprising Cyrtandra umbellifera, Briggisia longipes and Anna

mollifolia, and the monophyletic group is strongly supported by the bootstrap value (97).

The tribes Trichosporeae and Cyrtandreae represented respectively by Anna mollifolia and

Cyrtandra urnbellifera both evolved from the tribe Didymorcarpeae, which can explain why

many intermediate taxa exist among the three tribes. According to this, the present authors

suggest that the tribe Trichosporeae and the tribe Cyrtandreae be merged with the tribe

Didymocarpeae.

Key words: Cyrtandroideae, Internal transcrlbedspacers(ITS), Molecularphylogeny

汪小全,李振宇. rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用[J]. 中国科学院大学学报.

WANG Xiao-Quan, LI Zhen-Yu. The Application of Sequence Analysis of rDNA Fragment to the Systematic Study of the Subfamily Cyrtandroideae (Gesneriaceae)[J]. .

[1]	王超,王建波,施苏华,钟扬. 山羊草属异源多倍体物种核rDNA ITS区的进化[J]. 中国科学院大学学报, 2000, 38(3): 211-217.
[2]	张文驹,瞿礼嘉,高巍,顾红雅,陈章良,陈家宽. 用ITS序列确定小麦B基因组的可能供体间的关系[J]. 中国科学院大学学报, 1999, 37(5): 417-424.
[3]	王建波,张文驹,陈家宽. 核rDNA的ITS序列在被子植物系统与进化研究中的应用[J]. 中国科学院大学学报, 1999, 37(4): 407-416.
[4]	汪小全,邓峥嵘,洪德元. 铁破锣属的系统位置——ITS(nrDNA)序列证据[J]. 中国科学院大学学报, 1998, 36(5): 403-410.
[5]	俸宇星,汪小全,潘开玉,洪德元. rbc L基因序列分析对连香树科和交让木科系统位置的重新评价——兼论低等金缕梅类的关系[J]. 中国科学院大学学报, 1998, 36(5): 411-422.
[6]	李建华, A. Linn Bogle, Anita S. Klein, 潘开玉. 金缕梅科银缕梅属与帕罗堤属的亲缘关系——核糖体DNA ITS序列证据[J]. 中国科学院大学学报, 1997, 35(6): 481-493.
[7]	葛颂, B. A. Schaal, 洪德元. 用核糖体DNA的ITS序列探讨裂叶沙参的系统位置——兼论ITS片段在沙参属系统学研究中的价值[J]. 中国科学院大学学报, 1997, 35(5): 385-395.
[8]	汪小全, 韩英, 邓峥嵘, 洪德元. 松科系统发育的分子生物学证据[J]. 中国科学院大学学报, 1997, 35(2): 97-106.

rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用

The Application of Sequence Analysis of rDNA Fragment to the Systematic Study of the Subfamily Cyrtandroideae (Gesneriaceae)

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