柑桔种质资源谷氨酸草酰乙酸转氨酶同工酶研究

中国科学院大学学报

柑桔种质资源谷氨酸草酰乙酸转氨酶同工酶研究

钟广炎, 叶荫民, 陈竹生, 吴云伦

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-03-10 发布日期:1993-03-10
通讯作者: 钟广炎

A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources

Zhong Guang-yan, Ye Yin-min, Chen Zhu-sheng, Wu Yun-lun

Received:1900-01-01 Revised:1900-01-01 Online:1993-03-10 Published:1993-03-10
Contact: Zhong Guang-yan

摘要/Abstract

摘要：

本文分析了125份柑桔属、枳属、金柑属和指来檬属种质资源的谷氨酸草酰乙酸转氨酶同工

酶(GOT)。新发现了GOT-1的两个等位基因B和C。宽皮柑桔的GOT同工酶颇为一致(仅两个例

外)，表明可能有共同的起源。酸橙的GOT同工酶较复杂，可能有多种起源，其中朱栾和小红橙的起

源可能与宜昌橙或香橙有关。在单胚种柚中发现了6种GOT基因组合。宜昌橙存在着两种GOT同工

酶类型。本文讨论了甜橙、酸橙、葡萄柚、香橙、柠檬等种类的可能起源。

关键词: 柑桔属, 枳属, 金柑属, 指来檬属, GOT同工酶

Abstract:

The glutamate oxaloacetate transaminase isozymes (GOT) extracted

from 125 biotypes of Citrus and its relatives, Fortunella, Poncirus and Microcitrus

were analyzed using polyacrylamide gel electrophoresis to investigate the taxonomic

relationships among citrus plants. Besides all the isozymes reported before, two new

bands were detected and designated their putative alleles B and C in GOT-l.

Among pummelo cultivars wide variations were found. Most of the mandarins

were identical, having SS for GOI-1 and MM for GOT-2 except for

Zou-Pi-Gan and Yao-Gan, which both had FS at GOT-1 and might be hy-

brids. This finding suggests that all of the mandarins may have originated from a

common ancestor. Sour orange biotypes showed a considerable variation in GOT

isozymes. Most of the sour oranges in China were assumed to be hybrids between

pummelo and mandarin based on GOT isozyme patterns, but two biotypes,

Xiao-Hong-Cheng and Zhu-lan, had FS at GOT-1 and MB at GOT-2, which

strongly suggests that they be hybrids of pummelo and Ichang papeda because B

allele of GOT-2 occurs only in Ichang papeda and its close relatives Yuzu and

Ichang lemon. From this study Yuzu is assumed to be derived from hybridization

of Ichan, papeda and mandarin.

Key words: Citrus, Poncirus, Fortunella, Microcitrus, GOT isozymes

钟广炎, 叶荫民, 陈竹生, 吴云伦. 柑桔种质资源谷氨酸草酰乙酸转氨酶同工酶研究[J]. 中国科学院大学学报.

Zhong Guang-yan, Ye Yin-min, Chen Zhu-sheng, Wu Yun-lun. A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources[J]. .

[1]	郭天池, 叶荫民. 中国柑桔属(芸香科)一新种[J]. 中国科学院大学学报, 1997, 35(4): 353-355.
[2]	钟广炎, 叶荫民. 柑桔植物的数值分类学研究[J]. 中国科学院大学学报, 1993, 31(3): 252-260.
[3]	钟广炎, 叶荫民. 柑桔种质资源过氧化物酶同工酶分析[J]. 中国科学院大学学报, 1991, 29(5): 418-422.