收稿日期: 2010-04-20
网络出版日期: 2010-11-15
基金资助
国家自然科学基金(30572252,30670455)、中国科学院研究生院院长基金(O95101PY00)和王宽诚教育基金会项目资助
Inhibitory effects of strawberry extracts on animal fatty acid synthase
Received date: 2010-04-20
Online published: 2010-11-15
刘晓鑫 , 田维熙 , 马晓丰 . 草莓提取物对脂肪酸合酶及脂肪细胞的抑制作用[J]. 中国科学院大学学报, 2010 , 27(6) : 768 -777 . DOI: 10.7523/j.issn.2095-6134.2010.6.006
Fatty acid synthase (FAS) has been considered as a potential new therapeutic target for obesity. In the present study we show that the components of both strawberry ( Fragaria ananassa Duch) fruit dreg and strawberry leaves that extracted with 50% ethanol and partitioned by ethyl acetate are potent FAS inhibitors. The ethyl acetate fractions of strawberry fruit dregs (SF-AE) show noncompetitive inhibition with the substrate Mal-CoA, while the ethyl acetate fractions of strawberry leaves (SL-AE) show competitive inhibition with Ac-CoA. Based on the HPLC-MS analysis, we conclude that ellagic acid and quercetin are the efficient components. SF-AE and SL-AE could reduce lipids accumulation in 3T3-L1 preadipocyte with dose-dependence. These results may be of great value for application of strawberry extracts to obesity prevention and cure.
Key words： fatty acid synthase; strawberry; inhibitor; kinetic assay; anti-obesity
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