收稿日期: 2010-04-29
修回日期: 2010-05-07
网络出版日期: 2011-01-20
基金资助
中国科学院研究生院启动基金(055101FM03)资助
Effect of extracellular Ca2+ on osteoblast differentiation in MG-63 cells
Received date: 2010-04-29
Revised date: 2010-05-07
Online published: 2011-01-20
郑家强 , 杨建虹 . 细胞外钙离子对人成骨样细胞 MG-63分化的影响[J]. 中国科学院大学学报, 2011 , 28(1) : 57 -64 . DOI: 10.7523/j.issn.2095-6134.2011.1.009
In the present study we have examined effects of extracellular Ca2+ on osteoblast differentiation in MG-63 cells.Theresults showed that5.0mmol/L Ca2+ up-regulated the expression of CaSR , OC、ALP、Runx 2、IGF-1 ,and BMP 2 . However,these indicators decreased after treatment with blocking agents. In addition,5.0 and 10.0mmol/L Ca2+ increased phospho-ERK expression, but did not affect ERK.Therefore 5.0mmol/L Ca2+ up-regulated CaSR and then promoted osteoblast differentiation through ERK pathway in MG-63 cells.
Key words： Ca2+; differentiation; osteoblast; CaSR; ERK
[1] Brown E M,MacLeod R J.Extracellular calcium sensing and extracellular calcium signaling
[J].Physiol Rev,2001,81:239-297.
[2] MacLeod R J,Yano S,Chattopadhyay N,et al.Extracellular calcium-sensing receptor transactivates the epidermal growth factor receptor by a triple-membrane-spanning signaling mechanism
[J].Biochem Biophys Res Commun,2004,320:455- 460.
[3] Yamaguchi T,Kifor O,Chattopadhyay N,et al.Expression of extracellular calcium (Ca2+)-sensing receptor in the clonal osteoblast-like cell lines,UMR-106 and SAOS-2
[J].Biochem Biophys Res Commun,1998,243:753-757.
[4] Theman T A,Collins M T.The role of the calcium-sensing receptor in bone biology and pathophysiology
[J].Curr Pharm Biotechnol,2009,10:289-301.
[5] Yamaguchi T,Chattopadhyay N,Kifor O,et al.Activation of p42/44 and p38 mitogen-activated protein kinases by extracellular calcium-sensing receptor agonists induces mitogenic responses in the mouse osteoblastic MC3T3-E1 cell line
[J].Biochem Biophys Res Commun,2000,279:363-368.
[6] Muller P,Bulnheim U,Diener A,et al.Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells
[J].J Cell Mol Med,2008,12:281-291.
[7] Huang Z,Cheng S L,Slatopolsky E. Sustained activation of the extracellular signal-regulated kinase pathway is required for extracellular calcium stimulation of human osteoblast proliferation
[J].J Biol Chem,2001,276:21351-21358.
[8] Yamaguchi T, Chattopadhyay N, Kifor O, et al. Mouse osteoblastic cell line (MC3T3-E1) expresses extracellular calcium (Ca2+)-sensing receptor and its agonists stimulate chemotaxis and proliferation of MC3T3-E1 cells
[J].J Bone Miner Res,1998,13:1530-1538.
[9] Sugimoto T,Kanatani M,Kano J,et al.Effects of high calcium concentration on the functions and interactions of osteoblastic cells and monocytes and on the formation of osteoclast-like cells
[J].J Bone Miner Res,1993,8:1445-1452.
[10] Maeno S,Niki Y,Matsumoto H,et al.The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture
[J].Biomaterials,2005,26:4847- 4855.
[11] Guo J,Li H Z,Zhang W H,et al.Increased expression of calcium-sensing receptors induced by ox-LDL amplifies apoptosis of cardiomyocytes during simulated ischaemia/reperfusion
[J].Clin Exp Pharmacol Physiol,2010,37:e128-e135.
[12] Shen Y Y, Chen K,Xu N.Osteogenic capacity of human deciduous dental pulp stem cells in vitro
[J]. J South Med Univ, 2010, 30(1): 96-99 (in Chinese). 申元源,陈柯,许诺.人乳牙牙髓干细胞体外成骨特性的实验研究
[J]. 南方医科大学学报,2010,30(1):96-99.
[13] Matsumoto T,Nakamura S,Sasaki J I,et al.Effect of calcium ion concentrations on osteogenic differentiation and hematopietic stem cell niche-related protein expressions in osteoblasts
[J].Tissue Eng Part A,2010,16:2467-2473.
[14] Yamaguchi T,Sugimoto T.Impaired bone mineralization in calcium-sensing receptor (CaSR) knockout mice:the physiological action of CaSR in bone microenvironments
[J].Clin Calcium,2007,17:1567-1573.
[15] Chang W,Tu C,Chen T H,et al.The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development
[J].Sci Signal,2008,1(ra1).
[16] Huh Y J,Kim J M,Kim H,et al.Regulation of osteoclast differentiation by the redox-dependent modulation of nuclear import of transcription factors
[J].Cell Death Differ,2006,13:1138-1146.
[17] Enomoto H,Furuichi T,Zanma A,et al.Runx2 deficiency in chondrocytes causes adipogenic changes in vitro
[J].J Cell Sci,2004,117:417- 425.
[18] Nakashima K,de Crombrugghe B. Transcriptional mechanisms in osteoblast differentiation and bone formation
[J].Trends Genet,2003,19:458- 466.
[19] Zhang W X,Chen S L,Yao X L,et al.Effects of BMP-2 and FGF-2 on osteoblast differentiation of murine MSCs in vitro
[J].Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi,2008,24:1062-1065.
[20] Lee M H,Kim Y J,Kim H J,et al.BMP-2-induced Runx2 expression is mediated by Dlx5, and TGF-beta 1 opposes the BMP- 2 -induced osteoblast differentiation by suppression of Dlx5 expression
[J].J Biol Chem,2003,278:34387-34394.
[21] Zhang W,Lee J C,Kumar S,et al.ERK pathway mediates the activation of Cdk2 in IGF-1-induced proliferation of human osteosarcoma MG-63 cells
[J].J Bone Miner Res,1999, 14: 528-535.
[22] Ogata N,Kawaguchi H. Involvement of insulin and IGF-1 signaling molecules in bone metabolism
[J].Clin Calcium,2008,18: 614-622.
[23] Ge C,Xiao G,Jiang D,et al.Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development
[J].J Cell Biol,2007,176:709-718.
[24] Prasadam I,Friis T,Shi W,et al.Osteoarthritic cartilage chondrocytes alter subchondral bone osteoblast differentiation via MAPK signalling pathway involving ERK1/2
[J]. Bone,2010,46:226-235.
[25] Kim Y H,Kim J M,Kim S N,et al.p44/42 MAPK activation is necessary for receptor activator of nuclear factor-kappa ligand induction by high extracellular calcium
[J].Biochem Biophys Res Commun,2003,304:729-735.
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