收稿日期: 2011-11-09
修回日期: 2012-02-17
网络出版日期: 2013-01-15
基金资助
国家自然科学基金(20571085,20877099,20972183);国家十二五计划支撑项目(2012BAI37B03);国家科技部863项目(2008AA100801);湖南高等教育研究基金(06C068);中国科学院计算地球动力学重点实验室开放基金(06);北京大学天然药物及仿生药物国家重点实验室开放基金(K20120201)和中国科学院研究生院院长基金资助
Recognition and detection of target DNA using radio-labelled peptide nucleic acids
Received date: 2011-11-09
Revised date: 2012-02-17
Online published: 2013-01-15
王光伟 , 黄亮 , 向开祥 , 赵红 , 何裕建 . 以放射性标记的肽核酸对目标基因的识别与检测[J]. 中国科学院大学学报, 2013 , 30(1) : 1 -5 . DOI: 10.7523/j.issn.1002-1175.2013.01.001
The radio-111In3+ labelled peptide nucleic acid (PNA) was used as molecular probe and the pUC19-C1 plasmid which contains the target site of the PNA probe was constructed. The results show that PNA forms a stable double helix with not only the complementary single-stranded nucleic acid but also the plasmid in the presence of PNA opener. It is suggested that radio-labelled PNA probe can be used to detect target DNA in genes.
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