收稿日期: 2012-02-23
修回日期: 2012-03-15
网络出版日期: 2012-03-15
基金资助
国家自然科学基金(30670889,30771193)资助
Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation
Received date: 2012-02-23
Revised date: 2012-03-15
Online published: 2012-03-15
冯晓琴 , 徐峰 , 王嵬 , 刘利新 . 通过对免疫共沉淀技术的优化验证蛋白质弱相互作用[J]. 中国科学院大学学报, 2013 , 30(1) : 18 -23 . DOI: 10.7523/j.issn.1002-1175.2013.01.004
Co-immunoprecipitation is widely used to detect protein-protein interaction in physiological condition. The single-factor method was used to optimize some factors of co-immunoprecipitation, including component concentrations of cell-lysis buffer, antibody dosage, hCLP46 protein quantity, and cross-linking DSP. As a result, the weak interaction between hCLP46 (human CAP10-like protein 46) and ER chaperone calnexin is confirmed. The present work provides an important basis for further study of the protein-protein interaction.
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