收稿日期: 2012-03-16
修回日期: 2012-04-28
网络出版日期: 2012-04-28
基金资助
中国科学院知识创新工程重要方向项目——生命科学领域十二五基础前沿专项(KZCX2-EW-J-29)资助
Involvement of AtACO3 in Zn homeostasis
Received date: 2012-03-16
Revised date: 2012-04-28
Online published: 2012-04-28
李燕 , 王建武 , 谭金娟 , 冯珊珊 , 柴团耀 . 顺乌头酸酶AtACO3参与调控Zn稳态的研究[J]. 中国科学院大学学报, 2013 , 30(3) : 329 -333 . DOI: 10.7523/j.issn.1002-1175.2013.03.008
A full-length cDNA, designated as AtACO3, was isolated from Arabidopsis thaliana. Semi-quantitative RT-PCR revealed that AtACO3 was significantly activated under treatment with 200 μmol/L ZnCl2 for 1 hour while inhibited under treatment with 200 μmol/L CuCl2. To investigate the function of the N-terminus of AtACO3, the prokaryotic expression vector pET30a with a fragment of AtACO3 including 1632 bp from the initiation codon was constructed and successfully transformed E. coli. SDS-PAGE indicated that the recombinant N-terminus of AtACO3 was soluble and stable when expressed in E. coli. Additionally, the tolerance of E. coli against Zn2+ was found to be enhanced by expressing the N-terminus fragment of AtACO3.
Key words： Zn; Arabidopsis thaliana; aconitase
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