收稿日期: 2013-04-01
修回日期: 2013-04-19
网络出版日期: 2014-01-15
基金资助
国家自然科学基金（61173098）和中国科学院“十二五”基础前沿专项（KSCX2-EW-J-29）资助
Effects of nitric oxide on the biosynthesis of shikonin and its derivatives in callus of Arnebia euchroma
Received date: 2013-04-01
Revised date: 2013-04-19
Online published: 2014-01-15
郝鹤 , 叶和春 , 王红 . 一氧化氮对新疆紫草愈伤组织中紫草素及其衍生物合成的影响[J]. 中国科学院大学学报, 2014 , 31(1) : 46 -53 . DOI: 10.7523/j.issn.2095-6134.2014.01.008
Nitric oxide (NO) is an important signal molecule involved in the regulation of plant secondary metabolism. In this study, SNP (sodium nitroprusside) was utilized as the donor of nitric oxide to treat Arnebia euchroma callus. We find that the expression levels of the genes involved in the biosynthesis of shikonin and its derivatives, such as AePGT, AeGPPS, AeC4H, and Ae4CL, increase significantly in SNP-treated Red Strain callus tissues. Meanwhile, SNP treatment also increases the contents of some shikonin derivatives. The above-mentioned results indicate that exogenous NO increases the biosynthesis of shikonin and its derivatives by up-regulating the expression of the genes.
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