收稿日期: 2013-05-06
修回日期: 2013-05-23
网络出版日期: 2014-03-15
基金资助
国家自然科学基金（41176144）；海洋公益性行业科研专项（20120527）和山东省自然科学基金（JQ200914）资助
MSRF analyses of methylation differences between two Enteromorpha prolifera populations (attached and free-floating) in Yellow Sea
Received date: 2013-05-06
Revised date: 2013-05-23
Online published: 2014-03-15
应用甲基化敏感限制性指纹技术（MSRF），利用对DNA甲基化敏感与不敏感的限制性内切酶组合产生基因组的酶切多态性差异图谱. 发现漂浮浒苔可能在BstUI位点发生了甲基化增强的现象，推测表观遗传水平上的改变可能推动了漂浮浒苔对黄海自由漂浮生活的快速适应.
关键词： 浒苔; 表观遗传学; 甲基化; 甲基化敏感的限制性指纹技术
王萌 , 崔玉琳 , 王寅初 , 刘正一 , 秦松 . 基于MSRF的黄海漂浮浒苔与定生浒苔的甲基化差异分析[J]. 中国科学院大学学报, 2014 , 31(2) : 182 -186 . DOI: 10.7523/jssn.2095-6134.2014.02.007
The free-floating population of Enteromorpha prolifera, which results in the blooms in Yellow Sea, has remarkable phenotypic variations compared to the attached population. We applied methylation-sensitive restriction fingerprinting (MSRF) to E. prolifera for methylation polymorphism with methylation-sensitive enzyme digestion. Our results showed that the free-floating sample might be abnormally hypermethylated at BstUI sites. It is suggested that these changes at epigenetic level may promote the rapid adaptation of E. prolifera to free-floating lives in Yellow Sea.
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