收稿日期: 2002-12-30
修回日期: 2003-05-19
网络出版日期: 2004-01-10
基金资助
国家自然科学基金资助项目(40171092)
Methane Oxidation in Soil Profiles of Broad-Leaved/ Korean Pine Mixed Forest in Changbai Mountain
Received date: 2002-12-30
Revised date: 2003-05-19
Online published: 2004-01-10
采集长白山阔叶红松林不同深度土壤,在实验室条件下测定其对高低体积分数CH4的氧化和相关菌群的数量变化。结果表明,土壤氧化CH4的能力随深度变化明显;5 - 1 5cm土层具有最大CH4氧化活性,在 40 0 μL/LCH4体积分数下,此土层干重土壤CH4最大氧化速率可达3.3nmol·h- 1·g- 1 ;25cm以下土层基本没有CH4氧化活性;因 0 - 5cm土层土壤含有高质量浓度NH+4,抑制了CH4氧化菌的活性,所以此层土壤对CH4吸收能力下降。对微生物数量统计结果显示,5 - 1 0cm土层CH4氧化菌数量最大,CH4氧化菌对林土CH4氧化贡献大,硝化菌的作用很小。
梁战备 , 史奕 , 王琛瑞 , 岳进 . 长白山阔叶红松林不同深度森林土壤氧化CH4研究[J]. 中国科学院大学学报, 2004 , 21(1) : 71 -77 . DOI: 10.7523/j.issn.2095-6134.2004.1.011
Samples were collected from soil in different depths in the virgin broad leavedl Korean pine mixed forest in Changbai Mountain. Laboratory study on methane oxidation of forest soil revealed the vertical profiles of methane oxidation activity. Maximum methane oxidation occurred at the horizon located at 5 to 15cm below the soil surface. When incubated with 400 μL/ L methane,it had the highest uptake rate of 3.3 nmol.h- 1g- 1.Soil at horiwn below 25 cm had negligible capacity to oxidize methane.Methane-oxidizing capacity of surface soil ( 0-5 cm) decreased because of inhibition effect of high concentration NH4 on methanotrophs. The number of methanotrophs was greatest in soil at 5-10 cm below the surface.Methanotroph contributed more to methane oxidation than nitrifer did.
Key words： methane oxidation; vertical profile; methanotroph
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