收稿日期: 2003-01-09
修回日期: 2003-03-31
网络出版日期: 2004-01-10
基金资助
国家计委“九五”、“十五”重点攻关项目
Study on the Effect of Bacillus Megateriums in Two-Stage Fermentation of Vc
Received date: 2003-01-09
Revised date: 2003-03-31
Online published: 2004-01-10
在维生素C二步发酵中,Bacillusmegaterium作为伴生菌能有效促进Gluconobacteroxydans的生长和产VitaminC的前体 2 酮基 L 古龙酸(简称 2 KLG)。实验室利用低能离子对出发菌BP5 2进行诱变选育,获得一株B.megaterium突变体GB82,此突变体与G.oxydans组成的混合体系GAB,比原始混合体系 2 980产酸水平高 1 0个百分点以上。比较研究了GB82与原始B.megateriumBP5 2分泌的生物活性物质对产酸菌G.oxydans产 2 KLG的影响,据此提出此活性物质可能是某种蛋白,且此蛋白在极端条件下会部分失活的观点;并且通过RandomAmplifiedPolymorphicDNA(RAPD)分析揭示了低能离子注入造成的二者基因水平上的差异。
关键词： Bacillusmegaterium; Vc二步发酵; RAPD
吕树娟 , 王军 , 虞龙 , 谢传晓 , 谷运红 , 姚建铭 , 余增亮 . Bacillus Megaterium在Vc二步发酵中伴生活性初步研究[J]. 中国科学院大学学报, 2004 , 21(1) : 84 -89 . DOI: 10.7523/j.issn.2095-6134.2004.1.013
Bacillus megaterium,as a companion strain in two-stage fermentation of vitamin C,could spur Gluconobacter ox ydans to grow and produce 2- ket o-L-gulonic acid( 2- KL G) the precursor of Vitamin C.In lab a mutated B. megaterium GB82 was screened from the original strain BP52 by low-energy ion implantation.When GAB,a mixed fermentation system which was com bined with G.oxydans and GB82,yielded 2- KLG from L-sorbose, the conversion rate could reach as high as 96%,about 10% higher than 2980,a mixed fermentation system which was combined with G.ox ydans and BP52. Studied and compared the effect of the active substances secreted by GB82 and BP52 on the ability of G.oxydans to produce 2-KL G. Hereby, suggested that the active substances be some proteins and the proteins be deactivated partly under extreme conditions.By means of Random Amplified Poly morphic DNA ( RAPD),also analyzed the genomic differences between BP52 and GB82 which were caused by low-energyion im plant ation.
Key words： Bacillus megateriums; two-stage fermentation of Vc; RAPD
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