收稿日期: 2004-04-13
修回日期: 2004-06-28
网络出版日期: 2005-03-15
Establishment of Mathematic-Physical Models for Target Immobilization in High-Throughput Screening
Received date: 2004-04-13
Revised date: 2004-06-28
Online published: 2005-03-15
李松军 , 刘白玲 . 高通量药物筛选中靶分子固定化数学物理模型的建立[J]. 中国科学院大学学报, 2005 , 22(2) : 179 -186 . DOI: 10.7523/j.issn.2095-6134.2005.2.009
Based on the analysis of the basic process involved in high-throughput screening ( HTS), the present article discusses the possible effect of target immobilizat ion on the screening quality. Judged from intermolecular interactions, the actual process in HTS is more complicated than that in ideal situation. With more or less interactions electrostatic, hydrophobic or hydrogen-bonding can be involved in this process. With the induction derived from these interactions, the immobilization mode of target may be in monolayer or a more complicated mult-i layer form. In addit ion, this article presents the mathematic expressions related to the corresponding interaction forms, including target and target, target and microsphere, and bulk phase and surface phase as well. These expressions are expected to show a certain degree of usability in the future work.
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