收稿日期: 2004-03-18
修回日期: 2004-09-15
网络出版日期: 2005-07-15
基金资助
国家自然科学基金项目 (50447007)资助
The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity
Received date: 2004-03-18
Revised date: 2004-09-15
Online published: 2005-07-15
肖华娟 , 严萍 , 牟群 . 强脉冲电场致细胞膜电穿孔的实验研究[J]. 中国科学院大学学报, 2005 , 22(4) : 462 -466 . DOI: 10.7523/j.issn.2095-6134.2005.4.010
In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine Bisothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects.
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