收稿日期: 2002-01-13
修回日期: 2002-04-24
网络出版日期: 2002-07-18
基金资助
中国科学院“西部之光”资助项目:共轭亚油酸系列产品的研究与开发(200 2～2004年)
Quantitation of Conjugated Linoleic Acid by UV Spectrophotometry
Received date: 2002-01-13
Revised date: 2002-04-24
Online published: 2002-07-18
张亚刚 , 樊莉 , 马莉 , 文彬 , 吾满江·艾力 . 用紫外可见分光光度法测定共轭亚油酸的含量[J]. 中国科学院大学学报, 2002 , 19(4) : 436 -442 . DOI: 10.7523/j.issn.2095-6134.2002.4.017
Through absorbance measurement of conjugated linoleic acid, methy lester of conjugated linoleic acid and conjug ated linoleic acid trigly cide from 210 ～ 300 nm by UV-9100 spectrophotometer, absorbancy curve of measured samples at different concent ration is plotted.In the appropriate concentration range of samples, linear relationship of absorbancy and samples concent ration is presented by linear regression with Microcal Software Origin Version 4.0 system.The accurate concentration of conjugated linoleic acid is obtained with PE Autosystem XL-T ruboMass.Combined w ith reg ression constant data, emprical formula for the quantitation of conjugated linoleic acid by UV spect rophotometer is given.
[1] Iversen S A, Cawood M J, Madigan A M, et al.Identi fication of a diene conjugated component of human lipid asoct adeca-9, 11-dienoicacid.FEBS Lett, 1984, 171(2):320 ～ 324
[2] Si tunayake R D, Crump B J, Zezulka A V, et al.Use second-derivat ive spect roscopy to quantify conjugated dienes.Ann Clin Biochem,1990, 27 (3):258 ～ 266
[3] 曹栋, 史苏佳, 方伟旋.脱水蓖麻油的研究.中国油脂, 1999, 24(4):55 ～ 56
[4] Horning E C, Tu rner D A, Goldw ater W H, et al.Quant itative Analysi s of Fat ty Acids by Gas-Liquid Ch romat ography.J Lipid Res,1964, 5:20 ～ 27
[5] Werner A S, Luedecke L O, Shultz T D.Det ermination of conjugat ed linoleicacid in three cheddar-type cheeses:effects of cheese cul tures,processing,and aging.J Agric Food Chem, 1992, 40:1817 ～ 1821
[6] Fulk W K, Shorb M S.Production of an artifact during methanolysis of lipids by boron trifluoride-methanol.J Lipid Res, 1970, 11:276 ～277
[7] Dawidow icz E A, T hompson T E.Art ifacts produced by boron trifluoride methanolysis of a synthetic lectincont aining cyclopropane fattyacids(1-2-dihydrosterculoyl-3-snphosphatidyl choline).J Lipid Res, 1971, 12:636 ～ 637
[8] Luddy F E, Barf old R A, Riemenschneider R W.Direct conversion of lipid compotents to their fatty acid methyl esters.J Am Oil ChemSoc, 1960, 37:447 ～ 451
[9] Kramer J K G, Fellner V, Dugan M E R, et al.Evaluating acid and base catalyst s in the methyation of milk and rumen fatty acids with specialemphasis on conjugated dienes and total trans fatty acids.Lipids, 1997, 32:1219 ～ 1228
[10] Lorette N B, Brown Jr J H.Use of acetone dimethyl acetal in preparation of methyl ester.J Org Chem, 1959, 24:261 ～ 262
[11] Nalur Chandrasekaran Shantha, Eric And rew Decker, Bernhard Hennig.Comparison of methylation methods for the quantitation of conjugated linoleic acid isomers.J of AOAC International, 1993, 76:644 ～ 649
[12] Masao Yamasaki, Koji Kishihara, Ikuo Ikeda, et al.A recommended esterif icat ion method f or gas chromatographic measurement of conjugated linoleic acid.J Am Oil Chem Soc, 1999, 76:933 ～ 938
[13] 贝雷著.Y H Hui, 徐生庚, 裘爱泳, 倪培德等译.油脂化学与工艺学(第5版第2卷).北京:中国轻工业出版社, 2001.71
[14] Rossell J B, Allen J C, Hamilton R J.Rancidity in foods.London :Applied Science Publishers, 1987.21 ～ 45
/
| 〈 |
|
〉 |
