收稿日期: 2015-01-07
修回日期: 2015-05-20
网络出版日期: 2015-11-15
基金资助
国家自然科学基金(41271493)资助
Bioaccessibility of arsenic in lettuce and rape and its health risk assessment
Received date: 2015-01-07
Revised date: 2015-05-20
Online published: 2015-11-15
为评价砷污染土壤中种植的蔬菜对人体的健康风险,本研究在砷污染土壤中种植生菜和油菜,利用体外消化(in vitro)方法评估其生物可给性,计算食用砷污染蔬菜条件下人体砷的每日摄入量,评价蔬菜中砷对人体产生的健康风险.结果表明,生菜和油菜中砷的生物可给性均随着蔬菜中砷含量的上升而逐渐降低.在固液比为1:30的条件下生菜和油菜的生物可给性分别为29.13%~49.39%和27.13%~51.95%,在固液比为1:100的条件下生菜和油菜的生物可给性大幅提升,分别为42.39%~79.09%和37.40%~76.59%.在固液比为1:30的条件下,添加外源铁可使其生物可给性分别下降到22.24%~37.88%和23.36%~47.56%,添加外源钙则对其生物可给性几乎没有影响.食用砷污染土壤种植的蔬菜会使人体摄入的砷含量超出世界卫生组织规定的砷每日最高摄入量标准.在提升消化液中铁浓度0.006 7 mol·L-1的情况下,砷的生物可给性降低约6.89%~11.51%,食用含铁的食物能有效地减少人体对食物中砷的摄入.
王振洲 , 崔岩山 , 张震南 , 尹乃毅 , 蔡晓琳 , 都慧丽 . 生菜和油菜中砷的生物可给性及其对人体的健康风险评估[J]. 中国科学院大学学报, 2015 , 32(6) : 735 -742 . DOI: 10.7523/j.issn.2095-6134.2015.06.003
In the present study, in vitro method was used to assess human health risk of arsenic (As) in vegetables. Lettuce and rape were planted in the soil with different As pollutions, and bioaccessibility of As in the two vegetables was determined. Daily intake of As was also calculated, and the risk to human health was assessed. The results showed that the bioaccessibility of As decreased with the increasing concentration of As in the vegetables. Bioaccessibilities of As in the lettuce and rape were 29.13%-49.39% and 27.13%-51.95%, respectively, at solid-liquid ratio of 1:30. The values sharply rose to 42.39%-79.09% and 37.40%-76.59%, respectively, at solid-liquid ratio of 1:100. At the solid-liquid ratio of 1:30, the iron addition decreased the values to 22.24%-37.88% and 23.36%-47.56%, respectively, in the two vegetables, but the calcium addition made no change for the bioaccessibility of As. In raising the iron content of digestive juices by 0.0067 mol·L-1, bioaccessibility of As could reduce by about 6.89%-11.51%. Iron can effectively reduce As intake.
Key words： arsenic; lettuce; rape; bioaccessibility; health risk assessment
[1] Deba P S, Kunnath S S. Arsenic poisoning in West Bengal[J]. Science, 1996, 274:1 285-1 289.
[2] Nickson R, Mcarthur J, Burgess W, et al. Arsenic poisoning of Bangladesh groundwater[J]. Nature, 1998, 395:338.
[3] Chowdhury T R, Basu G K, Mandal B K. Arsenic poisoning in the Ganges delta[J]. Nature, 1999, 401:545-546.
[4] Karim M M. Arsenic in groundwater and health problems in Bangladesh[J]. Water Research, 2000, 34:304-310.
[5] Christen K. The arsenic threat worsens[J]. Environmental Science & Technology, 2001, 35:286-291.
[6] Pandey P K, Yadav S, Nair S, et al. Arsenic contamination of the environment:a new perspective from central-east India[J]. Environment International, 2002, 28:235-245.
[7] Xu H N, Xu J L. Cause of formation and distribution of arsenic in abnormal area of China[J]. Soil, 1996, 28:80-84.
[8] Hung C L H, So M K, Connell D W, et al. A preliminary risk assessment of trace elements accumulated in fish to the Indo-Pacific Humpback dolphin (Sousa chinensis) in the Northwestern waters of Hong Kong[J]. Chemosphere, 2004, 56(7):643-651.
[9] Ferreira B L, De Miguel E. Geochemistry and risk assessment of street dust in Luanda, Angola:a tropical urban environment[J]. Atmospheric Environment, 2005, 39(25):4 501-4 512.
[10] 谢正苗. 铅锌砷复合污染对水稻生长的影响[J]. 生态学报, 1994, 14(2):215-217.
[11] Berg M, Tran H C, Nguyen T C, et al. Arsenic contamination of ground water and drinking water in Vietnam:A human health threat[J]. Environment Science & Technology, 2001, 35:2 621-2 626.
[12] Ruby M V, Schoof R, Brattin E, et al. Advances in evaluating the oral bioavailability of inorganics in soil for use in human health risk assessment[J]. Environmental Science & Technology, 1999, 33(21):3 697-3 705.
[13] 朱永官. 农业环境中的砷及其对人体的健康风险[M]. 北京:科学出版社, 2013:120-122.
[14] Ruby M V, Davis A, Link T E, et al. Development of an in vitro screening test to evaluate the in vivo bioaccessibility of ingested mine-waste lead[J]. Environmental Science & Technology, 1993, 27(13):2 870-2 877.
[15] Ruby M V, Davis A, Schoof R, et al. Estimation of lead and arsenic bioavailability using a physiologically based extraction test[J]. Environmental Science & Technology, 1996, 30(2):422-430.
[16] Artursson P, Karlsson J. Correlation between oral drug absorption in humans and apparent drug pemeability coefficients in human intestinal epithelial (Caco-2) cells[J]. Biochemistry Biophysical Research Communications, 1991, 175(3):880-885.
[17] Yang J, Mosby D E, Casteel S W, et al. Lead immobilization using phosphoric acid in a smelter-contaminated soil[J]. Environmental Science & Technology, 2001, 35(17):3 553-3 559.
[18] 付瑾, 崔岩山. In vitro 系统评价胃肠液pH 及土液比对铅、镉、砷生物可给性的影响[J]. 农业环境科学学报, 2012, 31(2):245-251.
[19] 崔岩山, 陈晓晨. 土壤中镉的生物可给性及其对人体的健康风险评估[J]. 环境科学, 2010, 31(2):403-408.
[20] Tang X Y, Zhu Y G. Advances in in vitro tests in evaluating bioavailability of heavy metals in contaminated soil via oral intake[J]. Environ Health, 2004, 21(1):183-185.
[21] 王陇德. 中国居民营养与健康状况调查之一:2002 综合报告[M]. 北京:人民卫生出版社, 2005:19-32.
[22] 兰砥中, 雷鸣, 周爽, 等. 体外模拟实验法评价湘南某矿区大米中重金属的人体健康风险[J]. 农业环境科学学报, 2014, 33(10):1 897-1 903.
[23] 陈同斌, 宋波, 郑袁明, 等. 北京市蔬菜和菜地土壤砷含量及其健康风险分析[J]. 地理学报, 2006, 61(3):297-310.
[24] 蔡宝松, 陈同斌, 廖晓勇, 等. 土壤砷污染对蔬菜砷含量及食用安全性的影响[J]. 生态学报, 2004, 24(4):711-717.
[25] 白玲玉, 曾希柏, 胡留杰, 等. 外源二甲基砷对油菜生长及土壤中砷生物有效性的影响[J]. 应用生态学报, 2011, 22(2):437-441.
[26] 柳晓娟, 刘文菊, 林爱军, 等. 中草药砷含量特征及其健康风险初步评价[J].环境科学, 2010,31(12):3 036-3 042.
[27] Laparra J M. Bioavailability of inorganic arsenic in cooked rice:practical aspects for human health risk assessments[J]. Journal of Agricultural and Food Chemistry, 2005,53:8 829-8 833.
[28] Laparra J M, Vélez D, Montoro R, et al. Estimation of arsenic bioaccessibility in edible seaweed by an in vitro digestion method[J]. Journal of Agricultural and Food Chemistry, 2003, 51(20):6 080-6 085.
[29] 汪光, 李开明, 吕永龙, 等. 食品烹调处理过程中砷的浓度、形态和生物可给性研究进展[J]. 生态毒理学报, 2013, 8(2):132-137.
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