采用水热法合成纳米CeO2线，使用透射电子显微镜、X射线衍射仪和拉曼光谱仪对其进行表征。使用傅里叶变换红外光谱仪和紫外可见分光光度计对DNA与纳米CeO2线的相互作用进行分析，然后采用紫外可见分光光度法研究DNA对纳米CeO2线类氧化酶活性的影响，并对体系进行pH影响以及特异性实验研究。结果表明，DNA抑制纳米CeO2线的类氧化酶活性，这种抑制作用具有特异性。此工作将使纳米CeO2得到进一步的实际应用。
The CeO2 nanowires were synthesized by the hydrothermal method. The sample was characterized by transmission electron microscopy, X-ray diffractometer, and Raman spectrometer. Fourier transform infrared spectrometer and UV-Vis spectrophotometer were used to analyze the interaction between DNA and CeO2 nanowires. The effect of DNA on the oxidase mimetic activity of CeO2 nanowires was further studied by UV-Vis spectrophotometry. The impact of pH on the system and specificity experiment were also studied. The results show that DNA inhibits the oxidase activity of CeO2 nanowires, and the inhibition is specific. This work further promotes the practical application of CeO2 nanowires.
[1] Pirmohamed T, Dowding J M, Singh S, et al. Nanoceria exhibit redox state-dependent catalase mimetic activity[J]. Chemical Communications, 2010, 46(16):2736-2738.
[2] Heckert E G, Seal S, Self W T. Fenton-like reaction catalyzed by the rare earth inner transition metal cerium[J]. Environmental Science & Technology, 2008, 42(13):5014-5019.
[3] Heckert E G, Karakoti A S, Seal S, et al. The role of cerium redox state in the SOD mimetic activity of nanoceria[J]. Biomaterials, 2008, 29(18):2705-2709.
[4] Asati A, Santra S, Kaittanis C, et al. Oxidase-like activity of polymer-coated cerium oxide nanoparticles[J]. Angewandte Chemie International Edition, 2009, 48(13):2308-2312.
[5] Vernekar A A, Das T, Mugesh G. Vacancy-engineered nanoceria:enzyme mimetic hotspots for the degradation of nerve agents[J]. Angewandte Chemie International Edition, 2016, 55(4):1412-1416.
[6] Liu B W, Huang Z C, Liu J W. Boosting the oxidase mimicking activity of nanoceria by fluoride capping:rivaling protein enzymes and ultrasensitive F- detection[J]. Nanoscale, 2016, 8(28):13562-13567.
[7] Huang L J, Zhang W T, Chen K, et al. Facet-selective response of trigger molecule to CeO2 {110} for up-regulating oxidase-like activity[J]. Chemical Engineering Journal, 2017, 330:746-752.
[8] Cheng H J, Lin S C, Muhammad F, et al. Rationally modulate the oxidase-like activity of nanoceria for self regulated bioassays[J]. Acs Sensors, 2016, 1(11):1336-1343.
[9] Tana, Zhang M L, Li J, et al. Morphology-dependent redox and catalytic properties of CeO2 nanostructures:Nanowires, nanorods and nanoparticles[J]. Catalysis Today, 2009, 148(1):179-183.
[10] Yang Y S, Mao Z, Huang W J, et al. Redox enzyme-mimicking activities of CeO2 nanostructures:Intrinsic influence of exposed facets[J]. Scientific Reports, 2016, 6:35344.
[11] Sisubalan N, Ramkumar V S, Pugazhendhi A, et al. ROS-mediated cytotoxic activity of ZnO and CeO2 nanoparticles synthesized using the Rubia cordifolia L. leaf extract on MG-63 human osteosarcoma cell lines[J]. Environmental Science and Pollution Research, 2017(4):1-11.
[12] Xu C, Liu Z, Wu L, et al. Nucleoside triphosphates as promoters to enhance nanoceria enzyme-like activity and for single-nucleotide polymorphism typing[J]. Advanced Functional Materials, 2014, 24(11):1624-1630.
[13] Pautler R, Kelly E Y, Huang P J J, et al. Attaching DNA to nanoceria:regulating oxidase activity and fluorescence quenching[J]. Acs Applied Materials & Interfaces, 2013, 5(15):6820-6825.
[14] Zhang Y, Zhou K B, Zhai Y W, et al. Crystal plane effects of nano-CeO2 on its antioxidant activity[J]. Rsc Advances, 2014, 4(92):50325-50330.
[15] Singh S, Dosani T, Karakoti A S, et al. A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties[J]. Biomaterials, 2011, 32(28):6745-6753.
