考察土壤种类、暴露时间及铬[Cr（VI）]含量对蚯蚓体内SOD（superoxide dismutase）、CAT（catalase）和POD（peroxidase）3种抗氧化酶活性的影响。实验选取黑龙江、山西和江苏3个地区的土壤作为研究对象，并采用不同含量Cr（VI）对其进行40天老化。结果表明，不同土壤中SOD活性差异显著（P<0.05），Cr（VI）含量和暴露时间对SOD活性影响差异极显著（P<0.01）。与SOD活性差异性相似的是：不同土壤中CAT活性差异显著（P<0.05），Cr（VI）含量和暴露时间对CAT活性影响差异极显著（P<0.01）。而在不同土壤中，POD活性差异极显著（P<0.01），Cr（VI）含量对POD活性影响差异显著（P<0.05），暴露时间对POD活性影响差异极显著（P<0.01）。蚯蚓在黑龙江老化土中暴露初期，POD和SOD活性显著升高。在暴露28天的实验组中POD和SOD活性最高，相应的酶活性浓度可分别达对照组的2.03和1.25倍。同时随暴露时间延长，POD和SOD活性均受到显著抑制，而蚯蚓体内CAT活性在各处理中不发生显著变化。蚯蚓在江苏老化土中暴露初期，POD和SOD变化趋势与黑龙江老化土中各变化趋势相近。山西老化土中暴露初期，POD和CAT活性显著升高，随暴露时间延长，POD和CAT活性均受到显著抑制，但蚯蚓体内SOD活性在各处理中不发生显著变化。
Effects of the soil type, exposure time, and chromium (VI) concentration on antioxidant enzyme[SOD (superoxide dismutase), CAT (catalase), and POD (peroxidase)] activity of Eisenia fetida were investigated. The experimental soils were selected from Heilongjiang, Shanxi, and Jiangsu Provinces, and then were aged for 40 days. The results of the significance analysis showed a significant difference in SOD activity in different soils (P<0.05), and Cr(VI) concentration and exposure time showed an extremely significant difference in SOD activity (P<0.01). Similar to SOD activity, the significant difference was found in the CAT activities of different soils (P<0.05), and Cr(VI) concentration and exposure time showed extremely significant effects (P<0.01) on CAT activity. Whereas, POD activities of different soils were significantly different (P<0.01), Cr(VI) concentration has a significant effect on POD activity (P<0.05), and the effect of exposure time on POD activity was significantly different (P<0.01). In Heilongjiang's aging soil, the POD and SOD activities increased significantly in the early exposure. In the experimental group exposed for 28 days, the maximum POD and SOD concentrations were 2.03 and 1.25 times those of the control group, respectively. With the delay of exposure time, the activities of POD and SOD were significantly inhibited, while there was no significant change in CAT activity in E. fetida. In the early exposure of soil of Jiangsu, the change trends of POD and SOD with time were similar to those of soil of Heilongjiang. In the early exposure of soil of Shanxi, POD and CAT activities increased significantly, and with the delay of exposure time the enzyme activity was significantly inhibited, but the SOD activity showed no obvious change under different aged treatments.
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