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脱水诱导基因RD特性及功能

  • 龚束芳 ,
  • 初明洋 ,
  • 杨雅涵 ,
  • 乔坤 ,
  • 王金刚
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  • 东北农业大学园艺园林学院, 哈尔滨 150030

收稿日期: 2020-08-28

  修回日期: 2020-12-07

  网络出版日期: 2020-12-07

基金资助

国家自然科学基金(31972450)、黑龙江省自然科学基金(C2018021)和黑龙江省自然科学基金优秀青年项目(YQ2020C002)资助

Research of dehydration-inducible gene RD in characterization and function

  • GONG Shufang ,
  • CHU Mingyang ,
  • YANG Yahan ,
  • QIAO Kun ,
  • WANG Jin'gang
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  • College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China

Received date: 2020-08-28

  Revised date: 2020-12-07

  Online published: 2020-12-07

摘要

脱水诱导基因(Responsive to Dehydration,RD)是一类能够调节植物脱水的基因,对植物脱水胁迫具有一定耐受性,有些成员还对低温、高盐等非生物胁迫具有不同程度的响应。RD成员分属于不同家族,在结构和功能方面存在差异。归纳不同RD的结构组成、蛋白保守基序、调控机理以及应对生物以及非生物胁迫时的功能, 以及RD基因启动子区域中不同的顺式作用元件在基因应对非生物胁迫时发挥的作用,可为今后对RD基因的研究提供相关参考。

本文引用格式

龚束芳 , 初明洋 , 杨雅涵 , 乔坤 , 王金刚 . 脱水诱导基因RD特性及功能[J]. 中国科学院大学学报, 2022 , 39(2) : 154 -164 . DOI: 10.7523/j.ucas.2020.0054

Abstract

Responsive to dehydration (RD) is a class of genes that regulates dehydration in plants. They are functionally tolerant to plant dehydration, some of which are responsive to abiotic stresses such as low temperature and high salinity. However, they belong to different families, respectively, and have discrepancy in the structure and function. In this paper, the structural composition, conserved motif, regulatory mechanism, and the function in response to biotic and abiotic stress were summarized in different RDs, as well as the different cis-acting elements in the promoter region played a role in response to abiotic stress so as to provide relevant basis for future researches on RD.

参考文献

[1] Yamaguchi-shinozaki K, Koizumi M, Urao S, et al. Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein[J]. Plant and Cell Physiology, 1992, 33(3): 217-224.DOI:10.1093/oxfordjournals.pcp.a078243.
[2] 向旭,傅家瑞. 脱落酸应答基因的表达调控及其与逆境胁迫的关系[J]. 植物学通报, 1998, 15(3): 11-16.DOI:10.3969/j.issn.1674-3466.1998.03.003.
[3] Koizumi M, Yamaguchi-Shinozaki K, Tsuji H, et al. Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana[J]. Gene, 1993, 129(2): 175-182.DOI:10.1016/0378-1119(93)90266-6.
[4] Andeme Ondzighi C, Christopher D A, Cho E J, et al. Arabidopsis protein disulfide isomerase-5 inhibits Cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds[J]. Plant Cell, 2008, 20(8): 2205-2220.DOI:10.1105/tpc.108.058339.
[5] Yamada K, Matsushima R, Nishimura M, et al. A slow maturation of a Cysteine protease with a granulin domain in the vacuoles of senescing Arabidopsis leaves[J]. Plant Physiology, 2001, 127(4):1626-1634.DOI:10.1104/pp.010551.
[6] Nylander M, Svensson J, Palva E T, et al. Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana[J]. Plant Molecular Biology, 2001, 45(3): 263-279.DOI:10.1023/a:1006469128280.
[7] Gilmour S J, Artus N N, Thomashow M F. cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana[J]. Plant Molecular Biology, 1992, 18(1): 13-21.DOI:10.1007/BF00018452.
[8] Yamaguchi-Shinozaki K, Shinozaki K. Characterization of the expression of a desiccation-responsive rd 29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants[J]. Molecilar and General Genetics, 1993, 236(2/3): 331-340.DOI:10.1007/BF00277130.
[9] Horvath D P, McLarney B K, Thomashow M F. Regulation of Arabidopsis thaliana L. (Heyn) cor 78 in response to low temperature[J]. Plant Physiology, 1993, 103(4): 1047-1053.DOI:10.1104/pp.103.4.1047.
[10] Nordin K, Vahala T, Palva E T. Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh.[J]. Plant Molecular Biology, 1993, 21: 641-653.DOI:10.1007/BF00014547.
[11] Takahashi S, Katagiri T, Yamaguchi-Shinozaki K, et al. An Arabidopsis gene encoding a Ca2+-binding protein is induced by abscisic acid during dehydration[J]. Plant and Cell Physiology, 2000, 41(7): 898-903.DOI:10.1093/pcp/pcd010.
[12] Aubert Y, Vile D, Pervent M, et al. RD 20 , a stress-inducible caleosin, participates in stomatal control, transpiration and drought tolerance in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2010, 51(12): 1975-1987.DOI:10.1093/pcp/pcq155.
[13] Poxleitner M, Rogers S W, Lacey Samuels A L, et al. A role for caleosin in degradation of oil-body storage lipid during seed germination[J]. The Plant Journal, 2006, 47(6): 917-933.DOI:10.1111/j.1365-313X.2006.02845.x.
[14] Yamaguchi-Shinozaki K, Shinozaki K. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd 22 , a gene responsive to dehydration stress in Arabidopsis thaliana[J]. Molecular and General Genetics, 1993, 238(1/2): 17-25.DOI:10.1007/BF00279525.
[15] 郑磊. 西伯利亚蓼(Polygonum sibiricum) rd 22 基因的克隆、表达与功能验证[D]. 哈尔滨: 东北林业大学, 2007.
[16] 李慧玉, 林士杰, 王珊, 等. 柽柳rd 22 基因的序列分析及耐盐性研究[J]. 西北农林科技大学学报(自然科学版), 2010, 38(6): 95-101.DOI:10.13207/j.cnki.jnwafu.2010.06.022.
[17] 黄旭新, 侯佩臣, 丁明全, 等. 秋茄KcRD 22 基因的克隆与功能分析[J]. 基因组学与应用生物学, 2011, 30(4): 273-280.DOI:10.3969/j.issn.1674-568X.2011.04.003.
[18] Phillips K, Ludidi N. Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD 22 -like genes[J]. Scientific Reports, 2017, 7(1): 8821.DOI:10.1038/s41598-017-08976-x.
[19] 郑磊, 刘关君, 杨传平. 西伯利亚蓼rd 22 基因的克隆与序列分析[J]. 植物研究, 2007, 27(2): 212-217.DOI:10.3969/j.issn.1673-5102.2007.02.019.
[20] Fujita M, Fujita Y, Maruyama K, et al. A dehydration-induced NAC protein, RD 26 , is involved in a novel ABA-dependent stress-signaling pathway[J]. The Plant Journal, 2004, 39(6): 863-876.DOI:10.1111/j.1365-313X.2004.02171.x.
[21] Tran L S P, Nakashima K, Sakuma Y, et al. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter[J]. The Plant Cell, 2004, 16(9): 2481-2498.DOI:10.1105/tpc.104.022699.
[22] Reizer J, Reizer A, Saier M H. The MIP family of integral membrane channel proteins: sequence comparisons, evolutionary relationships, reconstructed pathway of evolution, and proposed functional differentiation of the two repeated halves of the proteins[J]. Critical Reviews in Biochemistry and Molecular Biology, 1993, 28(3): 235-257.DOI:10.3109/10409239309086796.
[23] Bozovic V, Svensson J, Schmitt J, et al. Dehydrins (LTI29, LTI30, and COR47) from Arabidopsis thaliana expressed in escherichia coli protect thylakoid membranes during freezing[J]. Journal of the Serbian Chemical Society, 2013, 78(8): 1149-1160.DOI:10.2298/JSC121127017B.
[24] Puhakainen T, Hess M W, Mäkelä P, et al. Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis[J]. Plant Molecular Biology, 2004, 54(5): 743-753.DOI:10.1023/B.PLAN.0000040903.66496.a4.
[25] Msanne J, Lin J S, Stone J M, et al. Characterization of abiotic stress-responsive Arabidopsis thaliana RD 29 A and RD29B genes and evaluation of transgenes[J]. Planta, 2011, 234(1): 97-107.DOI:10.1007/s00425-011-1387-y.
[26] Yamaguchi-Shinozaki K, Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress[J]. The Plant Cell, 1994, 6(2): 251-264.DOI:10.1105/tpc.6.2.251.
[27] Nakashima K, Shinwari Z K, Sakuma Y, et al. Organization and expression of two Arabidopsis DREB 2 genes encoding DRE-binding proteins involved in dehydration and high-salinity-responsive gene expression[J]. Plant Molecular Biology, 2000, 42(4): 657-665.DOI:10.1023/a:1006321900483.
[28] Narusaka Y, Nakashima K, Shinwari Z K, et al. Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd 29 A gene in response to dehydration and high-salinity stresses[J]. The Plant Journal, 2003, 34(2): 137-148.DOI:10.1046/j.1365-313X.2003.01708.x.
[29] Uno Y, Furihata T, Abe H, et al. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions[J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(21): 11632-11637.DOI:10.1073/pnas.190309197.
[30] Choi H I, Hong J H, Ha J O, et al. ABFs, a family of ABA-responsive element binding factors[J]. Journal of Biological Chemistry, 2000, 275(3): 1723-1730.DOI:10.1074/jbc.275.3.1723.
[31] Abe H, Yamaguchi-Shinozaki K, Urao T, et al. Role of Arabidopsis MYC and MYB homologs in drought and abscisic acid-regulated gene expression[J]. The Plant Cell, 1997, 9(10): 1859-1868.DOI:10.1105/tpc.9.10.1859.
[32] Iwasaki T, Yamaguchi-Shinozaki K, Shinozaki K. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis[J]. Molecular and General Genetics, 1995, 247(4): 391-398.DOI:10.1007/BF00293139.
[33] Urao T, Yamaguchi-Shinozaki K, Urao S, et al. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence[J]. The Plant Cell, 1993, 5(11): 1529-1539.DOI:10.1105/tpc.5.11.1529.
[34] Wang H M, Zhou L, Fu Y P, et al. Expression of an apoplast-localized BURP-domain protein from soybean (GmRD22) enhances tolerance towards abiotic stress[J]. Plant, Cell & Environment, 2012, 35(11): 1932-1947.DOI:10.1111/j.1365-3040.2012.02526.x.
[35] Hanana M, Deluc L, Fouquet R, et al. Identification et caractérisation d’un gène de réponse à la déshydratation rd 22 chez la vigne (Vitis vinifera L.)[J]. Comptes Rendus Biologies, 2008, 331: 569-578.DOI:10.1016/j.crvi.2008.05.002.
[36] Matus J T, Aquea F, Espinoza C, et al. Inspection of the grapevine BURP superfamily highlights an expansion of RD 22 genes with distinctive expression features in berry development and ABA-mediated stress responses[J]. PLoS One, 2014, 9(10):e110372.DOI:10.1371/journal.pone.0110372.
[37] Aubert Y, Leba L J, Cheval C, et al. Involvement of RD 20 , a member of caleosin family, in ABA-mediated regulation of germination in Arabidopsis thaliana [J]. Plant Signaling & Behavior, 2011, 6(4): 538-540.DOI:10.4161/psb.6.4.14836.
[38] Daniels M J, Mirkov T E, Chrispeels M J. The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP[J]. Plant Physiology, 1994, 106(4): 1325-1333.DOI:10.1104/pp.106.4.1325.
[39] Chaumont F, Loomis W F, Chrispeels M J. Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities[J]. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(12): 6202-6209.DOI:10.1073/pnas.94.12.6202.
[40] Huang R F, Zhu M J, Kang Y, et al. Identification of plasma membrane aquaporin in guard cells of Vicia faba and its role in stomatal movement[J]. Acta Botanica Sinica, 2002, 44(1): 42-48.
[41] Hanano A, Bessoule J J, Heitz T, et al. Involvement of the caleosin/peroxygenase RD 20 in the control of cell death during Arabidopsis responses to pathogens[J]. Plant Signaling & Behavior, 2015, 10(4):e991574.DOI:10.4161/15592324.2014.991574.
[42] Bernoux M, Timmers T, Jauneau A, et al. RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the ralstonia solanacearum PopP2 effector[J]. The Plant Cell, 2008, 20(8): 2252-2264.DOI:10.1105/tpc.108.058685.
[43] Lampl N, Alkan N, Davydov O, et al. Set-point control of RD21 protease activity by AtSerpin 1 controls cell death in Arabidopsis [J]. The Plant Journal, 2013, 74(3): 498-510.DOI:10.1111/tpj.12141.
[44] Lampl N, Budai-Hadrian O, Davydov O, et al. Arabidopsis AtSerpin 1 , crystal structure and in Vivo interaction with its target protease RESPONSIVE TO DESICCATION-21 (RD 21 )[J]. Journal of Biological Chemistry, 2010, 285(18): 13550-13560.DOI:10.1074/jbc.M109.095075.
[45] Shindo T, Misas-Villamil J C, Hörger A C, et al. A role in immunity for Arabidopsis cysteine protease RD 21 , the ortholog of the tomato immune protease C14[J]. PLoS One, 2012, 7(1):e29317.DOI:10.1371/journal.pone.0029317.
[46] Ormancey M, Thuleau P, van der Hoorn R A L, et al. Sphingolipid-induced cell death in Arabidopsis is negatively regulated by the papain-like cysteine protease RD 21 [J]. Plant Science, 2019, 280: 12-17.DOI:10.1016/j.plantsci.2018.10.028.
[47] Kamranfar I, Xue G P, Tohge T, et al. Transcription factor RD 26 is a key regulator of metabolic reprogramming during dark-induced senescence[J]. New Phytologist, 2018, 218(4): 1543-1557.DOI:10.1111/nph.15127.
[48] Jia H Y, Zhang S J, Ruan M Y, et al. Analysis and application of RD 29 genes in abiotic stress response[J]. Acta Physiologiae Plantarum, 2012, 34(4): 1239-1250.DOI:10.1007/s11738-012-0969-z.
[49] Cheong Y H, Sung S J, Kim B G, et al. Constitutive overexpression of the calcium sensor CBL 5 confers osmotic or drought stress tolerance in Arabidopsis[J]. Molecules and Cells, 2010, 29(2): 159-165.DOI:10.1007/s10059-010-0025-z.
[50] Li F, Han Y Y, Feng Y N, et al. Expression of wheat expansin driven by the RD 29 promoter in tobacco confers water-stress tolerance without impacting growth and development[J]. Journal of Biotechnology, 2013, 163(3): 281-291.DOI:10.1016/j.jbiotec.2012.11.008.
[51] 柳娜, 杨文雄, 王世红, 等. 拟南芥rd 29 A启动子在不同胁迫下GUS活性分析[J].甘肃农业科技, 2019(5): 44-50.DOI:10.3969/j.issn.1001-1463.2019.05.010.
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