医院产科门诊环境微生物组研究&#x0002A;

尹乃毅; 高慧敏; 熊仕茂; 武玥; 蒋喜艳; 肖鹏; 赵芳; 李锐; 蔡晓琳; 刘如铟; 崔岩山

doi:10.7523/j.ucas.2025.026

中国科学院大学学报 >

0 2025020 - 2025020

DOI: https://doi.org/10.7523/j.ucas.2025.026

医院产科门诊环境微生物组研究^*

尹乃毅 ,
高慧敏 ,
熊仕茂 ,
武玥 ,
蒋喜艳 ,
肖鹏 ,
赵芳 ,
李锐 ,
蔡晓琳 ,
刘如铟 ,
崔岩山

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1 中国科学院大学资源与环境学院,北京 101408;
2 中国科学院大学中丹学院,北京 101408

收稿日期: 2025-03-04

修回日期: 2025-04-22

网络出版日期: 2025-07-17

基金资助

^*国家自然科学基金（42277431）资助

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A Study on the Microbiome in the Obstetric Outpatient Environment of Hospital

YIN Naiyin ,
GAO Huimin ,
XIONG Shimao ,
WU Yue ,
JIANG Xiyan ,
XIAO Peng ,
ZHAO Fang ,
LI Rui ,
CAI Xiaolin ,
LIU Ruyin ,
CUI Yanshan

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1 College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China;
2 Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, China

Received date: 2025-03-04

Revised date: 2025-04-22

Online published: 2025-07-17

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摘要

医院环境中微生物群落结构和抗生素抗性基因（ARGs）分布对生命早期具有潜在影响。研究采集北京市某医院产科门诊35个灰尘样本,通过宏基因组测序系统分析环境中的微生物群落及ARGs。结果显示,样本中检测到17个微生物菌门,其中放线菌门、变形菌门和厚壁菌门为绝对优势类群,三者相对丰度总和达97.66%。不同位置的微生物存在显著差异,高接触位置的多样性较高。样本中检出182种ARGs和91种抗生素类型,其中万古霉素类和四环素类丰度为44.95%。通过分箱识别出ARG宿主对应的细菌共111种,痤疮皮杆菌和卷曲乳杆菌为主要贡献者。本研究为理解医院产科门诊环境中微生物群落和ARGs的分布提供了重要数据,并可为医院感染的防控策略提供科学依据。

关键词： 产科门诊; 宏基因组测序; 微生物群落; 病原菌; 抗生素抗性基因

本文引用格式

尹乃毅 , 高慧敏 , 熊仕茂 , 武玥 , 蒋喜艳 , 肖鹏 , 赵芳 , 李锐 , 蔡晓琳 , 刘如铟 , 崔岩山 . 医院产科门诊环境微生物组研究^*[J]. 中国科学院大学学报, 0 : 2025020 -2025020 . DOI: 10.7523/j.ucas.2025.026

Abstract

The structure of microbial communities and the distribution of antibiotic resistance genes (ARGs) in hospital environments may have potential impacts on early life. This study collected 35 dust samples from the obstetrics outpatient environment of a tertiary hospital in Beijing and analyzed the microbial communities and ARGs using metagenomic sequencing technology. The analysis revealed 17 microbial phyla in the samples, among which Actinobacteria, Proteobacteria, and Firmicutes emerged as the dominant groups, with their cumulative relative abundance reaching 97.66%. Significant differences in microbial presence were observed among various locations, with higher diversity found in high-contact areas. In total, 182 types of ARGs and 91 types of antibiotics were identified, with the cumulative abundance of vancomycin and tetracycline classes reaching 44.95%. Through binning, a total of 111 types of bacteria corresponding to ARG hosts were identified, among which Cutibacterium acnes and Lactobacillus curvatus are the main contributors. This study provides important data for understanding the distribution of microbial communities and ARGs in the hospital maternity outpatient environment and may offer a scientific basis for infection prevention and control strategies in hospitals.

Key words： obstetrics outpatient; metagenomic sequencing; microbial communities; pathogens; antibiotic resistance genes

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