本文介绍一种新的DNA序列的三维图形表示，它可以避免相应空间图形的交叉和重叠。该图形表示的好处还在于，它仅仅有两种需要计算的可能情况，而在其它的三维表示中，往往有三种需要计算的情况。究其应用，我们对两种可能产生的表示情况，通过计算十二种不同物种间编码序列对应的L/L矩阵产生的正规特征值，构造一个2-维向量来检测上述物种间的相似性与非相似性。

We outline a novel 3D graphical representation of DNA sequences, which can avoid crossing and overlapping of the corresponding spatial curve. And the advantage is that we can just consider two different conditions, but three conditions in other 3D representations. As application, we construct a 2-component vector whose components are the normalized leading eigenvalues of the L/L matrices associated with DNA sequence based on the two conditions, to examine the similarities/dissimilarities among the coding sequences of different species.