收稿日期: 2002-01-07
修回日期: 2002-03-22
网络出版日期: 2002-07-18
基金资助
国家自然科学基金资助项目(30170234)
Protective Ef fects of Glycerine on Mammal ian Cells in Ion Implantation
Received date: 2002-01-07
Revised date: 2002-03-22
Online published: 2002-07-18
冯慧云 , 余立祥 , 刘星海 , 吴李君 . 甘油对离子注入动物细胞的保护效应[J]. 中国科学院大学学报, 2002 , 19(4) : 372 -375 . DOI: 10.7523/j.issn.2095-6134.2002.4.006
The discovery of bio-ef fects induced by ion implantation has opened a novel branch in the field of radiation biology ———the low energy heavy ion biology.The experimental samples in the field have, however, been within the limits of plants and microbes because of the sample's vacuum-resistant ability.The mutation studying of mammalian cells with ion implantation hasn't been reported in the past years.The main reason for that is that it is difficult to keep mammalian cells alive in vacuum during ion implantation.In the present study, the tolerance of mammalian cells in vacuum was enhanced efficiently by pre-treatment with glycerin at low concent rat ion and proper treatment time, which provides a new way for the study on the biological effects of ion beam implantation in the mammalian cells.
Key words： low energy ions; AL cell; bio-effects; glycerine; protection
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