关键词: hCLP46, 骨髓增生异常综合征(MDS), CpG island, p15

Abstract: hCLP46, a novel gene, has been screened out from the cDNA library of MDS-AML patient’s CD34+ stem cell. In order to further study the biological characters of the gene, RT-PCR was used to test the tissue specificity of hCLP46 gene expression. The results revealed hCLP46 was expressed in adult brain, not in fetal liver. After being sequenced, hCLP46 gene was cloned into XbaI~BamHI sites of pcDNA3.1. pcDNA3.1-hCLP46-myc was further transient transfected into U937 cells to detect the mechanisms of cell proliferation. It was shown that the expression of p15 inducted by TGF-βwas down regulated .By reference to gene sequence, the bio-informatics approach was used to elucidate the biology function of hCLP46, the analysis showed that a classical “CpG island” was found in the promoter of the gene which implied that the gene might be participated in methylation pathway.

Key words: hCLP46, Myelodysplastic syndrome (MDS), CpG island, P15