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中国科学院大学学报 ›› 2010, Vol. 27 ›› Issue (6): 847-852.DOI: 10.7523/j.issn.2095-6134.2010.6.017

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拟南芥GH3基因家族启动子序列分析

孙涛1, 柴团耀1, 张玉秀2   

  1. 1. 中国科学院研究生院生命科学学院,北京 100049;
    2. 中国矿业大学(北京)化学与环境工程学院,北京 100083
  • 收稿日期:2009-12-14 发布日期:2010-11-15
  • 基金资助:

    国家高技术研究发展计划项目(2007AA021404, 2006AA10Z407)和转基因生物新品种培育科技重大专项(2009ZX08009-130B)资助 

Upstream promoter sequences of Arabidopsis GH3 gene family

SUN Tao1, CHAI Tuan-Yao1, ZHANG Yu-Xiu2   

  1. 1. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
    2. School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China
  • Received:2009-12-14 Published:2010-11-15

摘要:

GH3 基因家族是植物生长素早期响应基因家族之一.拟南芥 10个GH3 基因启动子序列分析表明:GH3 基因的转录起始位点一般在起始密码子上游65~145bp之内,TATA box大多分布在(-24)~(-40)bp区域.MDB和MatInspector分析显示,AtGH3 s基因的上游调控区域普遍存在组织和器官特异性表达元件、激素响应元件以及环境响应元件,表明GH3 基因的表达受到多因素调控;同时,基因芯片数据显示AuxREs对生长素的响应非常重要,但也可能存在其他的生长素响应元件.

关键词: GH3 基因, 启动子, 顺式作用元件

Abstract:

GH3 genes belong to a primary auxin-response gene family. The 10 promoter sequences of Arabidopsis GH3 genes were analyzed using bioinformatics method. The results show that the transcription start site of these genes is generally 65~145bp away from the start codon, and the TATA boxes are located in the (-24)-(-40)bp. MDB and MatInspector analyses show that most upstream regions of these GH3 genes contain the cis-elements required for tissue and organ-specific expression responding to phytohormones and external environment, indicating that the expressions of GH3 genes are strictly controlled by multi-factors. Gene chip data show that AuxREs is very important for GH3 genes in response to IAA treatment,but it is not the unique cis-element for auxin response.

Key words: GH3 gene, promoter sequence, cis-element

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