导数紫外光谱法研究pH诱导牛血清蛋白构象变化

doi:10.7523/j.issn.2095-6134.2011.1.003

中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (1): 12-18.DOI: 10.7523/j.issn.2095-6134.2011.1.003

导数紫外光谱法研究pH诱导牛血清蛋白构象变化

彭钢^1,2, 刘白玲¹, 赵春霞^1,2, 江正武^1,2

1. 中国科学院成都有机化学研究所,成都 610041;
2. 中国科学院研究生院,北京 100049

收稿日期:2010-03-31 修回日期:2010-05-17 发布日期:2011-01-20
基金资助:
国家自然科学基金(20974111)资助 

pH-induced conformational transitions of bovine serum albumin investigated by ultraviolet derivative spectroscopy

PENG Gang^1,2, LIU Bai-Ling¹, ZHAO Chun-Xia^1,2, JIANG Zheng-Wu^1,2

1. Chengdu Institute of Organic Chemistry,Chinese Academy of Sciences,Chengdu 610041,China;
2. Graduate University, Chinese Academy of Sciences,Beijing 100049,China

Received:2010-03-31 Revised:2010-05-17 Published:2011-01-20

摘要/Abstract

摘要：

采用二阶导数紫外光谱,研究了牛血清蛋白(BSA)中色氨酸(Trp)、酪氨酸(Tyr)和苯丙氨酸(Phe)3种芳香族氨基酸残基的精细吸收谱带,并结合其肽键的吸收谱带综合分析了BSA pH值在2.3~12区间的构象变化.研究结果表明,BSA在等电点(pH值 4.7)附近时构象变化较大;而在pH 值5.7~10区间,构象变化较小;在强酸(pH值2.3)和强碱(pH值12)环境中,BSA构象变化非常明显.同时还发现,BSA质量浓度也在一定程度上影响其构象.

关键词: 牛血清蛋白, 构象变化, 导数紫外光谱

Abstract:

The fine absorption bands of three aromatic amino acid residues,Tryptophan (Trp),Tyrosine (Tyr),and Phenylalanine (Phe),of bovine serum albumin (BSA) were studied by ultraviolet second derivative spectroscopy.The conformation transitions of BSA at pH 2.3-12 were analyzed along with the absorption spectrum of the peptide bonds.It is observed that there exist obvious conformation transitions around the isoelectric point (pH 4.7) of BSA.The transitions are subtle between pH 5.7 and pH 10 but distinct in strong acidity(pH 2.3) and alkaline(pH 12) environments. Meanwhile,the concentration of BSA has certain effect on the conformation transition.

Key words: bovine serum albumin(BSA), conformational transition, ultraviolet derivative spectroscopy

中图分类号:

Q512.1

彭钢, 刘白玲, 赵春霞, 江正武. 导数紫外光谱法研究pH诱导牛血清蛋白构象变化[J]. 中国科学院大学学报, 2011, 28(1): 12-18.

PENG Gang, LIU Bai-Ling, ZHAO Chun-Xia, JIANG Zheng-Wu. pH-induced conformational transitions of bovine serum albumin investigated by ultraviolet derivative spectroscopy[J]. , 2011, 28(1): 12-18.

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导数紫外光谱法研究pH诱导牛血清蛋白构象变化

pH-induced conformational transitions of bovine serum albumin investigated by ultraviolet derivative spectroscopy

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