HPLC法同时测定氧化槐果碱、槐定碱和苦参碱

doi:10.7523/j.issn.2095-6134.2011.3.018

中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (3): 398-401.DOI: 10.7523/j.issn.2095-6134.2011.3.018

HPLC法同时测定氧化槐果碱、槐定碱和苦参碱

吴志娇¹, 赵红¹, 薛英¹, 胡新明¹, 毛一卿², 谭焕然²

1. 中国科学院研究生院, 北京 100049;
2. 北京大学, 北京 100191

收稿日期:2010-05-17 修回日期:2010-09-06 发布日期:2011-05-15
基金资助:
国家科技支撑计划(2009BAK61B01)、国家重点基础研究发展计划(2007CB935900)和中国科学院长春应用化学研究所电分析化学开放研究实验室基金(SKLEAC2010005)资助 

Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC

WU Zhi-Jiao¹, ZHAO Hong¹, XUE Ying¹, HU Xin-Ming¹, MAO Yi-Qing², TAN Huan-Ran²

1. Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
2. Peking University, Beijing 100191, China

Received:2010-05-17 Revised:2010-09-06 Published:2011-05-15

摘要/Abstract

摘要：

建立了同时测定氧化槐果碱、槐定碱和苦参碱的HPLC(高效液相色谱)方法.用VP-ODS(维普C18柱)150mm × 4.6 mm, 5 μm 色谱柱,柱温40 ℃,流动相为0.01mol/L pH 8.5磷酸盐缓冲溶液-甲醇(40 ∶60),流速 1.0 mL/min,紫外检测器波长215 nm.结果表明,3种生物碱在确定的色谱条件下得到很好的分离,且在所选质量浓度范围20~200 mg/L 内线性关系良好,回收率范围97.2%~102%.

关键词: 氧化槐果碱, 槐定碱, 苦参碱, 高效液相色谱

Abstract:

An HPLC method for simultaneous determination of oxysophocarpine, sophoridine, and matrine was proposed. VP-ODS column (150 mm × 4.6 mm, 5 μm) was used in the experiment. Column temperature was 40 ℃, mobile phase was 0.01 mol/L phosphate buffer solution (pH 8.5)-methanol (40 ∶60), UV detection wavelength was 215nm, and flow rate was 1.0 mL/min. The results indicated that the three kinds of alkaloid were separated effectively and showed good linearity within the selected concentration range.

Key words: oxysophocarpine, sophoridine, matrine, HPLC

中图分类号:

R917

吴志娇, 赵红, 薛英, 胡新明, 毛一卿, 谭焕然. HPLC法同时测定氧化槐果碱、槐定碱和苦参碱[J]. 中国科学院大学学报, 2011, 28(3): 398-401.

WU Zhi-Jiao, ZHAO Hong, XUE Ying, HU Xin-Ming, MAO Yi-Qing, TAN Huan-Ran. Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC[J]. , 2011, 28(3): 398-401.

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HPLC法同时测定氧化槐果碱、槐定碱和苦参碱

Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC

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