喜旱莲子草花序正反向SSH cDNA文库的构建及EST分析

doi:10.7523/j.issn.2095-6134.2012.6.007

中国科学院大学学报 ›› 2012, Vol. ›› Issue (6): 767-774.DOI: 10.7523/j.issn.2095-6134.2012.6.007

喜旱莲子草花序正反向SSH cDNA文库的构建及EST分析

王兰英^1,2, 陈洁^1,2, 刘卫¹

1. 中国科学院华南植物园, 广州 510650;
2. 中国科学院研究生院, 北京 100049

收稿日期:2011-09-13 修回日期:2011-12-23 发布日期:2012-11-15
通讯作者: 刘卫
基金资助:
国家重点基础研究发展规划(973)项目(2009CB119204)和国家自然科学基金(30530160)资助

Construction and EST analysis of suppression subtractive hybridization library of Alternanthera philoxeroides inflorescence

WANG Lan-Ying^1,2, CHEN Jie^1,2, LIU Wei¹

1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
2. Graduate University, Chinese Academy of Sciences, Beijing 100049, China

Received:2011-09-13 Revised:2011-12-23 Published:2012-11-15

摘要/Abstract

摘要： 利用SSH技术构建了喜旱莲子草雌雄同花和雄蕊心皮化花序的正反向基因表达的cDNA文库. 对文库部分克隆分析发现,EST功能涉及花性别发育、细胞代谢、生长发育、抗性适应、转录调控、酶调节、叶绿体和胞质基因、细胞内组件等8种不同功能类型.共筛选到8类与已知调控花性别发育相关的EST,如HD基因家族、MADS-box基因家族、线粒体基因、CCLS4家族蛋白等.

关键词: 喜旱莲子草, 雄蕊心皮化, SSH文库, EST分析

Abstract: Functions of the SSH cDNA library of two different inflorescences of hermaphrodite and pistillate of Alternanthera philoxeroides were sorted into eight different categories, involving the development of sex differentiation of flowers, the process of metabolism, cytoplasmic gene etc. Sex differentiation ESTs point to eight kinds of gene families, including homeobox ZIP, MADS-box, CCLS4 protein family, mitochondria male-fertile, and cytoplasmic male-sterility genes.

Key words: Alternanthera philoxeroides, stamen-pistillody, SSH library, EST analysis

中图分类号:

Q781

王兰英, 陈洁, 刘卫. 喜旱莲子草花序正反向SSH cDNA文库的构建及EST分析[J]. 中国科学院大学学报, 2012, (6): 767-774.

WANG Lan-Ying, CHEN Jie, LIU Wei. Construction and EST analysis of suppression subtractive hybridization library of Alternanthera philoxeroides inflorescence[J]. , 2012, (6): 767-774.

参考文献

[1] Mitchell C H, Diggle P K. The evolution of unisexual flowers: morphological and functional convergence results from diverse developmental transitions[J]. American Journal of Botany, 2005, 92(7):1068-1076.
[2] Chen Z. Feminization of stamens of alternanthera Philoxeroides grisebich[J].Acta Botanica Sinica,1964,12(2):133-135(in Chinese). 陈倬.空心苋雄蕊雌化的现象[J].植物学报,1964,12(2):133-135.
[3] Molliard M M. DE l'hermaphrodisme chez LA mercuriale ET LE chavre[J]. Revue Generale DE Botanique,1898:321-334.
[4] Sasakuma T, Ohtsuka I. Cytoplasmic effects of Aegilops species having D genome in wheat. I. Cytoplasmic differentiation among five species regarding pistillody induction[J]. Seiken Ziho,1979,27(28):59-65.
[5] Yamada K,Saraike T,Shitsukawa N,et al. Class D and B sister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.) [J]. Plant Molecular Biology,2009,71(1):1-14.
[6] Saraike T, Shitsukawa N,Yamamoto Y,et al. Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat[J]. Planta,2007,227(1): 211-221.
[7] Diatchenko L, Lau Y F, Campbell A P, et al. Suppression subtractive hybridization:a method for generating differentially regulated or tissue-specific cDNA probes and libraries[J]. Proceedings of the National Academy of Sciences, 1996,93(12):6025-6030.
[8] Kim J Y, Chung Y S, Ok S H, et al. Characterization of the full-length sequences of phospholipase A2 induced during flower development1[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression,1999,1489(2-3): 389-392.
[9] Suzuki Y,Nijhout H F. Evolution of a polyphenism by genetic accommodation[J]. Science,2006,311:650-652.
[10] Geng Y P,Pan X Y,Xu C Y, et al. Plasticity and ontogenetic drift of biomass allocation in response to varying resource availabilities in perennial herbs: a case study of Alternanthera philoxeroides[J].Ecological Research, 2007,22(2): 255-260.
[11] Allen J O, Fauron C M, Minx P, et al. Comparisons among two fertile and three male-sterile mitochondrial genomes of maize[J]. Genetics,2007,177(2):1173-1192.
[12] Jennifer Li-Pook-Than, Catherine Carrillo, Nima Niknejad,et al. Relationship between RNA splicing and exon editing near intron junctions in wheat mitochondria[J]. Physiologia Plantarum, 2007, 129(1): 23-33.
[13] Li Q J, Xu B, Chen X Y, et al. The effects of increased expression of an Arabidopsis HD-ZIP gene on leaf morphogenesis and anther dehiscence[J]. Plant Science,2007,173(5):567-576.
[14] Lü L L,Sun G M,Liu Y G,et al. Progress of LEAFY homologous gene study[J]. Occidentalia Sinica, 2011,31(1):197-203(in Chinese). 吕玲玲,孙光明,刘玉革,等.LEAFY同源基因研究进展[J].西北植物学报,2011,31(1):197-203.
[15] Weigel D. The genetics of flower development: from floral induction to ovule morphogenesis[J]. Annual Review of Genetics,1995,29(1):19-39.
[16] Goodrich J,Puangsomlee P,Marta M,et al. A Polycomb-group gene regulates homeotic gene expression in Arabidopsis[J]. Nature,1997,386(6):44-51.
[17] Mayama T,Ohtsubo E,Tsuchimoto S. Isolation and expression analysis of Petunia CURLY LEAF-like genes[J]. Plant and Cell Physiology,2003,44(8):811-819.
[18] Barbacar N,Hinnisdaels S,Farbos I, et al. Isolation of early genes expressed in reproductive organs of the dioecious white campion (Silene latifolia) by subtraction cloning using an asexual mutant[J]. The Plant Journal,1997,12(4):805-817.
[19] Xu K Y,Ye W H,Duan X W,et al. Physiological adaptation of Alternanthera philoxeroides under water stress condition induced by polyethylene glycol[J]. Journal of Zhejiang University: Agricl & Life Scil, 2004,30(3): 271-277(in Chinese). 许凯扬,叶万辉,段学武,等.PEG诱导水分胁迫下喜旱莲子草的生理适应性[J].浙江大学学报: 农业与生命科学版,2004,30(3):271-277.
[20] Velasco R,Zharkikh A,Troggio M,et al. A high quality draft consensus sequence of the genome of a heterozygous grapevine variety[J]. PLoS One,2007,2(12): e1326.
[21] Cai H,Wei C L,Chen N. Chromosome karyotype characters of biological invasion in Alternanthera philoxeroides[J]. Chinese Journal of Tropical Crops,2009, 30(004):530-534(in Chinese). 蔡华,韦朝领,陈妮.生物入侵种喜旱莲子草的染色体核型特征[J].热带作物学报,2009,30(004):530-534.
[22] Geng Y P,Zhang W J,Li B,et al. Phenotypic pla sticity and inva siveness of alien plants[J]. Biodiversity Science,2004,12(4):447-455(in Chinese). 耿宇鹏,张文驹,李博,等.表型可塑性与外来植物的入侵能力[J].生物多样性,2004, 12(4):447-455.
[23] Kay S H,Haller W T. Evidence for the existence of distinct alligatorweed biotypes[J]. Journal of Aquatic Plant Management,1982,20:37-41.
[24] Ye W H,Li J,Cao H L,et al. Genetic uniformity of Alternanthera philoxeroides in South China[J]. Weed Research,2003,43(4):297-302.
[25] Geng Y P,Pan X Y,Xu C Y,et al. Phenotypic plasticity of invasive Alternanthera philoxeroides in relation to different water availability,compared to its native congener[J].Acta Oecologica,2006,30(3):380-385.
[26] Liu W. Phenotypic differentiation is associated with gender plasticity and its responsive delay to environmental change in Alternanthera philoxeroides-Phenotypic differentiation in alligator weed[J]. PLoS One,2011, 6(11): e27238.
[27] Schlichting C D,Pigliucci M. Control of phenotypic plasticity via regulatory genes[J].The American Naturalist,1993,142(2):366-370.

喜旱莲子草花序正反向SSH cDNA文库的构建及EST分析

Construction and EST analysis of suppression subtractive hybridization library of Alternanthera philoxeroides inflorescence

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

访问统计

联系我们