一种用于β2受体兴奋剂筛查的胶束电动色谱新方法

doi:10.7523/j.issn.2095-6134.2015.04.009

中国科学院大学学报 ›› 2015, Vol. 32 ›› Issue (4): 482-489.DOI: 10.7523/j.issn.2095-6134.2015.04.009

一种用于β₂受体兴奋剂筛查的胶束电动色谱新方法

孟祥英¹, 索兴梅², 丁永胜¹

1. 中国科学院大学生命科学学院, 北京 100049;
2. 中央民族大学信息工程学院, 北京 100081

收稿日期:2014-09-09 修回日期:2015-01-05 发布日期:2015-07-15
通讯作者: 丁永胜
基金资助:
Supported by the National Natural Sciences Foundation of China (21075135) and the Chinese Academy of Sciences (KSCX2-EW-J-29)

A new method for screening of β₂-agonists by micellar electrokinetic chromatography

MENG Xiangying¹, SUO Xingmei², DING Yongsheng¹

1. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
2. School of Information Engineering, Minzu University of China, Beijing 100081, China

Received:2014-09-09 Revised:2015-01-05 Published:2015-07-15
Supported by:
Supported by the National Natural Sciences Foundation of China (21075135) and the Chinese Academy of Sciences (KSCX2-EW-J-29)

摘要/Abstract

摘要： 建立一种快速、简单、有效的胶束电动色谱技术分别检测猪饲料、猪肝、猪肉和猪尿中的5种β₂受体兴奋剂.获得优化后的分离缓冲液为15 mmol/L SDS、15 mmol/L硼酸钠、pH=10、以及毛细管温度为25 ℃.在优化条件下,5种β₂受体兴奋剂在18 min内完成分离,在0.1~40 μg/mL浓度范围内具有良好的线性关系(R² > 0.999),其检出限(S/N≥3)在0.05~0.08 μg/mL范围之间.西马特罗,莱克多巴胺,克伦特罗具有较好的回收率(n=3),分别为95.8%,93.1%,95.0%;而沙丁胺醇和特布他林的回收率较低,分别为52.3%和69.4%.被测物的峰面积和迁移时间的相对标准偏差分别小于4.86%和 1.25%.利用该方法对5种不同猪饲料、猪肝、猪肉和猪尿样品中的沙丁胺醇,特布他林,西马特罗,莱克多巴胺和克伦特罗进行了筛查.

关键词: β₂受体兴奋剂, 胶束电动色谱, 筛查

Abstract: A method for the determination of five β₂-agonists in pig feed, liver, meat, and urine samples by micellar electrokinetic chromatography was developed. The separation and detection conditions were optimized in terms of SDS concentration, running buffer pH and concentration, capillary temperature. Under the optimized conditions (15 mmol/L borate, pH=10, 15 mmol/L SDS, and 25 ℃), all five analytes in the spiking samples were separated and identified within 18 min. Good linearity (R²>0.999) was obtained for the analytes in the range of 0.1-40 μg/mL with the limit of detection (S/N ≥ 3) in the range of 0.05-0.08 μg/mL. The satisfactory recoveries of cimaterol, ractopamine and clenbuterol were obtained in the range of 93.1%-95.8%, whereas the recoveries of salbutamol and terbutaline were at 52.3% and 69.4%, respectively. The relative standard deviations of peak area and migration time of the analytes were less than 4.86% and 1.25%, respectively. The method was successfully applied to monitor the presence of the selected β₂-agonists in pig feed, liver, meat and urine samples.

Key words: β₂-agonist, micellar electrokinetic chromatography, screening

中图分类号:

孟祥英, 索兴梅, 丁永胜. 一种用于β₂受体兴奋剂筛查的胶束电动色谱新方法[J]. 中国科学院大学学报, 2015, 32(4): 482-489.

MENG Xiangying, SUO Xingmei, DING Yongsheng. A new method for screening of β₂-agonists by micellar electrokinetic chromatography[J]. , 2015, 32(4): 482-489.

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一种用于β₂受体兴奋剂筛查的胶束电动色谱新方法

A new method for screening of β₂-agonists by micellar electrokinetic chromatography

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