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中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (1): 57-64.DOI: 10.7523/j.issn.2095-6134.2011.1.009

• 论文 • 上一篇    下一篇

细胞外钙离子对人成骨样细胞 MG-63分化的影响

郑家强, 杨建虹   

  1. 中国科学院研究生院,北京 100049
  • 收稿日期:2010-04-29 修回日期:2010-05-07 发布日期:2011-01-20
  • 基金资助:

    中国科学院研究生院启动基金(055101FM03)资助 

Effect of extracellular Ca2+ on osteoblast differentiation in MG-63 cells

ZHENG Jia-Qiang, YANG Jian-Hong   

  1. Graduate University, Chinese Academy of Sciences, Beijing 100049,China
  • Received:2010-04-29 Revised:2010-05-07 Published:2011-01-20

摘要:

研究不同浓度钙离子对人成骨样细胞MG-63分化的影响.结果表明, 5.0mmol/L Ca2+促进 CaSR、ALP、OC、Runx 2、IGF-1和BMP2 mRNA表达,而加入CaSR和ERK抑制剂后,则下调 ALP、OC、Runx 2、IGF-1和BMP2 mRNA表达.不同浓度Ca2+处理后,磷酸化-ERK(p-ERK)蛋白表达明显增加.因此,5.0mmol/L Ca2+通过上调CaSR基因的表达,激活ERK通路,促进MG-63成骨样细胞的分化.

关键词: 钙离子, 分化, 成骨细胞, CaSR, ERK

Abstract:

In the present study we have examined effects of extracellular Ca2+ on osteoblast differentiation in MG-63 cells.Theresults showed that5.0mmol/L Ca2+ up-regulated the expression of CaSR , OC、ALP、Runx 2、IGF-1 ,and BMP 2 . However,these indicators decreased after treatment with blocking agents. In addition,5.0 and 10.0mmol/L Ca2+ increased phospho-ERK expression, but did not affect ERK.Therefore 5.0mmol/L Ca2+ up-regulated CaSR and then promoted osteoblast differentiation through ERK pathway in MG-63 cells.

Key words: Ca2+, differentiation, osteoblast, CaSR, ERK

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