DNA对纳米CeO2线类氧化酶活性的影响

doi:10.7523/j.issn.2095-6134.2019.02.016

中国科学院大学学报 ›› 2019, Vol. 36 ›› Issue (2): 275-279.DOI: 10.7523/j.issn.2095-6134.2019.02.016

DNA对纳米CeO₂线类氧化酶活性的影响

杨丁丁, 姚鑫

中国科学院大学化学科学学院, 北京 100049

收稿日期:2017-12-19 修回日期:2018-03-29 发布日期:2019-03-15
通讯作者: 姚鑫
基金资助:
国家自然科学基金（21271184）、973项目（2014CB931900）和中国科学院科学仪器发展项目（ZDKYYQ20170001）资助

Effect of DNA on the oxidase mimetic activity of CeO₂ nanowires

YANG Dingding, YAO Xin

School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2017-12-19 Revised:2018-03-29 Published:2019-03-15

摘要/Abstract

摘要： 采用水热法合成纳米CeO₂线，使用透射电子显微镜、X射线衍射仪和拉曼光谱仪对其进行表征。使用傅里叶变换红外光谱仪和紫外可见分光光度计对DNA与纳米CeO₂线的相互作用进行分析，然后采用紫外可见分光光度法研究DNA对纳米CeO₂线类氧化酶活性的影响，并对体系进行pH影响以及特异性实验研究。结果表明，DNA抑制纳米CeO₂线的类氧化酶活性，这种抑制作用具有特异性。此工作将使纳米CeO₂得到进一步的实际应用。

关键词: 纳米CeO₂线, DNA, 紫外可见分光光度法, 类氧化酶活性, 抑制作用

Abstract: The CeO₂ nanowires were synthesized by the hydrothermal method. The sample was characterized by transmission electron microscopy, X-ray diffractometer, and Raman spectrometer. Fourier transform infrared spectrometer and UV-Vis spectrophotometer were used to analyze the interaction between DNA and CeO₂ nanowires. The effect of DNA on the oxidase mimetic activity of CeO₂ nanowires was further studied by UV-Vis spectrophotometry. The impact of pH on the system and specificity experiment were also studied. The results show that DNA inhibits the oxidase activity of CeO₂ nanowires, and the inhibition is specific. This work further promotes the practical application of CeO₂ nanowires.

Key words: CeO₂ nanowires, DNA, UV-Vis spectrophotometry, oxidase, inhibition

中图分类号:

杨丁丁, 姚鑫. DNA对纳米CeO₂线类氧化酶活性的影响[J]. 中国科学院大学学报, 2019, 36(2): 275-279.

YANG Dingding, YAO Xin. Effect of DNA on the oxidase mimetic activity of CeO₂ nanowires[J]. , 2019, 36(2): 275-279.

参考文献

[1] Pirmohamed T, Dowding J M, Singh S, et al. Nanoceria exhibit redox state-dependent catalase mimetic activity[J]. Chemical Communications, 2010, 46(16):2736-2738.
[2] Heckert E G, Seal S, Self W T. Fenton-like reaction catalyzed by the rare earth inner transition metal cerium[J]. Environmental Science & Technology, 2008, 42(13):5014-5019.
[3] Heckert E G, Karakoti A S, Seal S, et al. The role of cerium redox state in the SOD mimetic activity of nanoceria[J]. Biomaterials, 2008, 29(18):2705-2709.
[4] Asati A, Santra S, Kaittanis C, et al. Oxidase-like activity of polymer-coated cerium oxide nanoparticles[J]. Angewandte Chemie International Edition, 2009, 48(13):2308-2312.
[5] Vernekar A A, Das T, Mugesh G. Vacancy-engineered nanoceria:enzyme mimetic hotspots for the degradation of nerve agents[J]. Angewandte Chemie International Edition, 2016, 55(4):1412-1416.
[6] Liu B W, Huang Z C, Liu J W. Boosting the oxidase mimicking activity of nanoceria by fluoride capping:rivaling protein enzymes and ultrasensitive F^- detection[J]. Nanoscale, 2016, 8(28):13562-13567.
[7] Huang L J, Zhang W T, Chen K, et al. Facet-selective response of trigger molecule to CeO₂ {110} for up-regulating oxidase-like activity[J]. Chemical Engineering Journal, 2017, 330:746-752.
[8] Cheng H J, Lin S C, Muhammad F, et al. Rationally modulate the oxidase-like activity of nanoceria for self regulated bioassays[J]. Acs Sensors, 2016, 1(11):1336-1343.
[9] Tana, Zhang M L, Li J, et al. Morphology-dependent redox and catalytic properties of CeO₂ nanostructures:Nanowires, nanorods and nanoparticles[J]. Catalysis Today, 2009, 148(1):179-183.
[10] Yang Y S, Mao Z, Huang W J, et al. Redox enzyme-mimicking activities of CeO₂ nanostructures:Intrinsic influence of exposed facets[J]. Scientific Reports, 2016, 6:35344.
[11] Sisubalan N, Ramkumar V S, Pugazhendhi A, et al. ROS-mediated cytotoxic activity of ZnO and CeO₂ nanoparticles synthesized using the Rubia cordifolia L. leaf extract on MG-63 human osteosarcoma cell lines[J]. Environmental Science and Pollution Research, 2017(4):1-11.
[12] Xu C, Liu Z, Wu L, et al. Nucleoside triphosphates as promoters to enhance nanoceria enzyme-like activity and for single-nucleotide polymorphism typing[J]. Advanced Functional Materials, 2014, 24(11):1624-1630.
[13] Pautler R, Kelly E Y, Huang P J J, et al. Attaching DNA to nanoceria:regulating oxidase activity and fluorescence quenching[J]. Acs Applied Materials & Interfaces, 2013, 5(15):6820-6825.
[14] Zhang Y, Zhou K B, Zhai Y W, et al. Crystal plane effects of nano-CeO₂ on its antioxidant activity[J]. Rsc Advances, 2014, 4(92):50325-50330.
[15] Singh S, Dosani T, Karakoti A S, et al. A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties[J]. Biomaterials, 2011, 32(28):6745-6753.

DNA对纳米CeO₂线类氧化酶活性的影响

Effect of DNA on the oxidase mimetic activity of CeO₂ nanowires

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

访问统计

联系我们

[1]	卜新雅, 陈露, 阚玉贺, 雷华军, 何裕建, 封禄田, 吴丽. 手性氨基酸对DNA链式聚合反应的影响[J]. 中国科学院大学学报, 2021, 38(3): 306-313.
[2]	黄须啟, 李晓毅. DNA与B-PDEAEA自组装过程的分子动力学模拟[J]. 中国科学院大学学报, 2018, 35(4): 487-491.
[3]	姜波田维熙齐桂年. 四川边茶提取物对脂肪酸合酶的抑制作用[J]. 中国科学院大学学报, 2007, 24(3): 291-299.
[4]	迟锐高随祥. DNA序列的3D图形表示（英文）[J]. 中国科学院大学学报, 2007, 24(3): 280-286.
[5]	李平, 汪秉宏. DNA序列分析法在金融数据时间序列中的应用[J]. 中国科学院大学学报, 2003, 20(2): 200-204.
[6]	胡适宜,袁宗飞. 旋花科植物雄性细胞的细胞质DNA存在状况及其系统学意义[J]. 中国科学院大学学报, 2002, 40(6): 511-516.
[7]	张亚刚, 樊莉, 马莉, 文彬, 吾满江·艾力. 用紫外可见分光光度法测定共轭亚油酸的含量[J]. 中国科学院大学学报, 2002, 19(4): 436-442.