Abstract: Effect of galactomannan oligosaccharides (GMOS) on intestinal microglora and bacterial community in newly-weaning pigs by denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal RNA. Lincomycin and GMOS included in the diet appeared to have changed PCR-DGGE profiles of cecal microbiota compared to the control diet. The DNA bands of colonic microbiota were more than that of ileal microbiota, and the DNA bands of day 14 were more than that of day 0 post-weaning in ileal, but the bands of day 14 were as much as that of day 0. There were some same bands among different lanes, it meant that Lactobacillus salivarius, Ruminococcus forques and E.coli 0517:H7 existed in every treatment in ileal but Staphylococcus aureus, Lactobacillus salivarius, Ruminococcus forques, Bacillus subtilis and Clostridium perfringens in colon and the different bands showed that each had different microbiota. Similarities between DGGE profiles most were 20%~50%, except that of control and 0.2%GMOS in ileal were 80%. It meant that each treatment had its own microbiota profiles.

Key words: galactomannan oligosaccharides, weaning pigs, microbiota