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部分丛生竹同工酶的研究

方伟, 童再康, 许世亮   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1992-05-18 发布日期:1992-05-18
  • 通讯作者: 方伟

A Study on Isozyme from Some Caespitose Bamboos

Fang Wei, Tong Zai-Kang, Xu Shi-Liang   

  • Received:1900-01-01 Revised:1900-01-01 Online:1992-05-18 Published:1992-05-18
  • Contact: Fang Wei

摘要:

本文采用聚丙烯(月+先)胺凝胶电泳法测定了5属28种竹叶的谷氨酸草酰乙酸转氨酶(GOT),过氧化物酶(POD)和酯酶(EST)三种同工酶。测定结果表明,GOT同工酶比较稳定,对于竹子分属有较好的意义;POD与EST同工酶种间分化大,可作为竹子分种的参考依据。根据三种同工酶的酶谱及相似系数分析,结合经典分类,对丛生竹分类中存在的疑难问题提出了一些参考意见。

关键词: 丛生竹, 同工酶, 相似系数

Abstract:

The peroxidase, estrase and glutamic-oxalacetic transaminase isozymes (POD.
EST and GOT) in leaves of twenty-eight species belonging to 5 genera were analysed by
means of polyacrylamide gel electrophoresis. The results show as follows: The zymograms of
glutamic oxaiacetic transaminase isozyme are generally stable and therefore useful in distin-
guishing genera. The POD and EST isozymes vary among species and may be used as
reference basis for distinguishing species. Based on the zymograms of three enzymes and
similarty coefficients analysis, combined with classic systematic data authors suggest that
isoenzymatic data be valuable in solving some systematic problems of caespitose bamboos.

Key words: Caespitose bamboos, Isozyme, Similar coefficient