应用hprt和CD59基因研究辐射旁效应的产生及机理

doi:10.7523/j.issn.2095-6134.2006.6.005

中国科学院大学学报 ›› 2006, Vol. 23 ›› Issue (6): 752-758.DOI: 10.7523/j.issn.2095-6134.2006.6.005

应用hprt和CD59基因研究辐射旁效应的产生及机理

张蕾蕾; 吴李君; 韩伟; 陈少鹏; 鲍凌志; 赵烨; 余增亮

中国科学院等离子体物理研究所离子束生物工程学重点实验室，安徽合肥1126信箱，230031

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2006-11-15

Studies of the Radiation Induced Bystander Effects on Hprt and CD59 Genes of Human-hamster Hybrid AL Cells

ZHANG Lei-Lei, WU Li-Jun, HAN Wei, CHEN Shao-Peng, BAO Ling-Zhi, ZHAO Ye, YU Zeng-Liang

Key Laboratory of Ion Beam Bioengineering of Chinese Academy of Scie+nces,
Institute of Plasma Physics, Hefei 230031,China

Received:1900-01-01 Revised:1900-01-01 Published:2006-11-15

摘要/Abstract

摘要： 本研究将辐射后的Hprt-AL细胞与未辐射的Hprt+AL细胞共培养，通过加有次黄嘌呤氨甲基喋呤胸腺嘧啶（HAT）的培养基选择性杀死被辐射的Hprt-AL细胞，探讨了损伤信号在辐射与未辐射细胞间的传导及其机理。研究发现受辐射的Hprt-AL细胞能将损伤信号传导给未辐射的Hprt+AL细胞，导致其CD59基因位点突变率显著性增加。通过观察DMSO和Lindane对α粒子照射诱发的细胞旁效应的抑制作用发现，在受辐射的共培养实验组中，Lindane 能显著提高未辐射细胞的存活率，降低其突变率；DMSO仅能提高其存活率，但对其突变无明显影响，显示细胞间通讯在α粒子照射诱发细胞存活及基因突变旁效应中起重要作用。

关键词: 辐射诱导旁效应, 细胞存活和基因突变效应, 活性氧自由基, 细胞间间隙连接通讯

Abstract: To investigate signal transduction between irradiated cells and unirradiated cells and its potential mechanism, unirradiated hprt+ AL cells were co-cultured with irradiated hprt- AL cells which were then killed by HAT selected medium. Results show that irradiated hprt- AL cells could transmit damage signals to unirradiated hprt+ AL cells and increase the mutation fraction at the CD59 locus. Free radical scavenger DMSO and intercellular communication inhibitor Lindane significantly increase the survival of unirradiated cells while only Lindane treatment is able to suppress the CD59 mutagenicity in unirradiated cells. These results indicate that GJIC plays an important role in the process of RIBE.

中图分类号:

Q813

张蕾蕾, 吴李君, 韩伟, 陈少鹏, 鲍凌志, 赵烨, 余增亮. 应用hprt和CD59基因研究辐射旁效应的产生及机理[J]. 中国科学院大学学报, 2006, 23(6): 752-758.

ZHANG Lei-Lei, WU Li-Jun, HAN Wei, CHEN Shao-Peng, BAO Ling-Zhi, ZHAO Ye, YU Zeng-Liang. Studies of the Radiation Induced Bystander Effects on Hprt and CD59 Genes of Human-hamster Hybrid AL Cells[J]. Journal of University of Chinese Academy of Sciences, 2006, 23(6): 752-758.

应用hprt和CD59基因研究辐射旁效应的产生及机理

Studies of the Radiation Induced Bystander Effects on Hprt and CD59 Genes of Human-hamster Hybrid AL Cells

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