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中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (4): 481-488.DOI: 10.7523/j.issn.2095-6134.2011.4.009

• 论文 • 上一篇    下一篇

肿瘤抑制因子Caliban相互作用蛋白的筛选和验证

王喆1, 张洁2, 赵宇亮2, 秘晓林2   

  1. 1. 中国科学院研究生院材料与光电技术学院, 北京 100049;
    2. 中国科学院高能物理研究所, 北京 100049
  • 收稿日期:2010-06-07 修回日期:2010-09-28 发布日期:2011-07-15
  • 基金资助:

    国家自然科学基金(30871388)资助 

Identification and functional study of tumor suppressor Caliban interacting proteins

WANG Zhe1, ZHANG Jie2, ZHAO Yu-Liang2, BI Xiao-Lin2   

  1. 1. College of Materials Science and Opto-Electronic Technology, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
    2. Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-06-07 Revised:2010-09-28 Published:2011-07-15

摘要:

为了研究Caliban的肿瘤抑制机制,通过酵母双杂交的方法筛选得到了4个新的Caliban相互作用蛋白:Bip2、Uba2、RpS27A和CG7182.它们的功能涉及转录调节、肿瘤抑制和泛素化修饰等过程.通过免疫共沉淀证实了Bip2与Caliban在体外结合,并发现在果蝇体内过表达Bip2可以抑制 p 53 的转录.这些结果为研究Caliban的肿瘤抑制功能提供了基础.

关键词: 肿瘤抑制因子, Caliban, 酵母双杂交, Bip2

Abstract:

Caliban is a tumor suppressor. To understand its inherent mechanisms, we screened Caliban interacting proteins using the yeast two-hybrid system. We found four new Caliban interacting proteins, Bip2, Uba2, RpS27A, and CG7182. Moreover, over-expression of transcriptional factor Bip2 suppresses transcription of p 53. The tumor suppression function of p53 could be regulated through new mechanisms involving Bip2 and Caliban.

Key words: tumor suppressor, Caliban, yeast two hybrid, Bip2

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