10种披碱草属植物的RAPD分析及其系统学意义

中国科学院大学学报

10种披碱草属植物的RAPD分析及其系统学意义

周永红,郑有良,杨俊良,颜济,贾继增

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-09-10 发布日期:1999-09-10
通讯作者: 周永红

Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YAN Ji, JIA Ji-Zeng

Received:1900-01-01 Revised:1900-01-01 Online:1999-09-10 Published:1999-09-10
Contact: ZHOU Yong-Hong

摘要/Abstract

摘要：

利用随机扩增多态性DNA(RAPD)技术分析了10种披碱草属植物，即：Elymus sibiricus L.，E．

caninus(L．)L¨E．lanceolatus(Scribner et Smith)Gould，E．nutans Griseb，E．dahuricus Turcz，，

E．tangutorum(Nevski)Hand．-Mazz., E．brachyaristatus ALöve，E.submuticus(Keng)Keng f.，E．

cylidricus(Franch)Honda和E．excelsus Turcz. 。对34个OPRON公司十聚体随机引物进行多态性筛

选，25个(75．53％)能产生多态性。16个引物产生的136个DNA片断，用于计算种间Nei氏遗传相似

性系数分析，在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明：(1)四倍体和六倍体

物种各自聚为一支，它们之间的亲缘关系较远；(2)E．sibiricus和E. caninus；亲缘关系较近，支持把

Roegneria caninus (L．)Nevski归入Elymus；(3)E．nutans和E．dahuricus亲缘关系密切；(4)形态相

似、地理分布一致的E．brachyaristatus和E．submuticus存在着一定程度的核苷酸序列的差异，它们与

E．nutans和E．dahuricus有一定的亲缘关系；(5)E．excelsus 与 E．cylindricus的亲缘关系较近，它们

又与E．tangutorum有亲缘关系；(6)RAPD结果与形态学和细胞学等分析结果基本一致，RAPD分析

方法将为Elymus系统分类提供DNA水平上丰富的资料。

关键词: 披碱草属, RAPD分析, 亲缘关系, 染色体组, 形态学

Abstract:

Ten species of Elymus (Poaceae: Triticeae) were analyzed using random ampli-

lied polymorphic DNA (RAPD) markers. Thirty-four decamer oligonucleotide random

primers from Opron Technologies were used for polymorphic selection. 25 (73.53%) pro-

duced polymorphic products. A total of 136 bands amplified from 16 primers were selected

for RAPD analyses. The data were used to generate Nei's similarity coefficients and to con-

struct a dendrogram using UPGMA in NTSYS programs. The result showed that: (1)

Three tetraploid species, E. sibiricus L., E. caninus (L.) L. and E. lanceolatus (Scrib-

ner et Smith) Gould, were clustered in one group, while seven hexaploid species, E. nu-

tans Griseb., E. dahuricusTurcz., E. brachyaristatus A Löve, E. submuticus (Keng)

Keng f., E. tangutorum (Nevski)Hand.-Mazz., E. excelsus Turcz. and E. cylindricus

(Franch) Honda, were clustered in another group. The relationship between the tetraploid

and the hexaploid species was remote; (2) E. caninus was closely related to E. sibiricus.

This result supported that Roegneria canina (L.) Nevski was treated as Elymus caninus;

(3) E . nutans was closely related to E. dahuricus ; (4) E.brachyaristatus and E.sub-

muticus were morphologically similar and sympatric in distribution, but there were certain

differences between them in the nucleotide sequences. Both of them were related to E. nu-

tans and E. duhuricus; (5) E. excelsus was closely related to E. cylindricus, and they

were clustered with E. tangutorum; (6) RAPD results are basically comparable with those

obtained from studies on morphology and cytology. It is a useful supplementary method for

assessing the genetic relationships among Elymus species.

Key words: Elymus, RAFD ahalysis, Phylogenetic relationship, Genome, Morphology

周永红,郑有良,杨俊良,颜济,贾继增. 10种披碱草属植物的RAPD分析及其系统学意义[J]. 中国科学院大学学报.

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YAN Ji, JIA Ji-Zeng. Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA[J]. .

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