三种三尖杉属植物越冬期光合器的功能和膜蛋白成分的比较研究

中国科学院大学学报

三种三尖杉属植物越冬期光合器的功能和膜蛋白成分的比较研究

马红, 张正东, 李良璧, 马桂芝, 翟小京, 周佩珍

(中国科学院植物研究所，北京)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-12-18 发布日期:1986-12-18
通讯作者: 马红

A Comparative Study on Function and Membrane Proteins of Chloroplasts of Three Species of Cephalotaxus in Late Winter

Ma Hong, Zhang Zheng-Dong, Li Liang-Bi, Ma Gui-Zhi, Zhai Xiao-Jing, Zhou Pei-Zhen

(lnstitute of Botany, Academia Sinica. Beijing)

Received:1900-01-01 Revised:1900-01-01 Online:1986-12-18 Published:1986-12-18
Contact: Ma Hong

摘要/Abstract

摘要：

本文对三尖杉属中三种植物即：三尖杉Cephalotaxus fortunei，粗榧C.sinensis 和柱

冠日本粗榧C.harringtonia cv．Fastigiata越冬期的光合器功能进行了研究。研究结果表明

这三种常绿三尖杉植物越冬时叶绿体的光合放氧活性受到抑制，但光系统II中心的活性和镁

离子调节光能分配的功能存在。说明其越冬特性与松柏科植物类似。

叶绿体膜的低温荧光测定结果表明三种植物的F₆₈₅,F₆₉₅和F₇₃₅的比率各不相同。用SDS—

聚丙烯酰胺凝胶电泳分析叶绿体膜蛋白，发现柱冠日本粗榧的多肽数与三尖杉和粗榧有明显

差异。以上特征可能有助于分类或系统位置的研究。

关键词: 三尖杉属, 光系统I, 光系统II, 可变荧光, 低温荧光, 叶绿体膜多肽

Abstract:

Three species of evergreen Cephalotaxus (C. fortunei Hook. f., C. sinensis Li and

C. harringtonia cv. Fastigiata) were used as materials to study some functional properties of

chloroplasts. It is found that the oxygenevolving capacity of the chloroplasts from these plants

is inhibited but partial reaction of PS-II and effect of Mg²⁺ on energy distribution between

two photosystems are detectable during the winter. Seasonal effects on the functional properties

of chloroplasts from evergreen Cephalotaxus are similar to that of conifer chloroplasts.

The ratio between F₆₈₅, F₆₉₅ and F₇₃₅ of fluorescence emission spectra at 77°K of ch-

loroplasts is different among these three species. It is found by using SDS-PAGE that the num-

ber of polypeptide resolved from thylakoid membrane of C. harringtonia cv. Fastigiata sub-

stantially differs from that of C. fortunei Hook.f. and C. sinensis Li. The result shows that the

fluorescence emission spectrum feature and polypeptide composition of thylakoid membrane may

be used as a tool for systematics of the genus Cephalotaxus.

Key words: Cephalotaxus, Photosystem I, Photosystem II, variable fluorescence, low temperature fluorescene, chloroplast polypeptide

马红, 张正东, 李良璧, 马桂芝, 翟小京, 周佩珍. 三种三尖杉属植物越冬期光合器的功能和膜蛋白成分的比较研究[J]. 中国科学院大学学报.

Ma Hong, Zhang Zheng-Dong, Li Liang-Bi, Ma Gui-Zhi, Zhai Xiao-Jing, Zhou Pei-Zhen. A Comparative Study on Function and Membrane Proteins of Chloroplasts of Three Species of Cephalotaxus in Late Winter[J]. .

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