利用RAPD分子标记评价仲彬草属的种间关系

中国科学院大学学报 ›› 2000, Vol. 38 ›› Issue (6): 515-521.

利用RAPD分子标记评价仲彬草属的种间关系

周永红,郑有良,杨俊良,颜济,贾继增

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2000-11-10
通讯作者: 周永红

Relationships among Kengyilia species assessed by RAPD markers

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YEN Chi, JIA Ji-Zeng

Received:1900-01-01 Revised:1900-01-01 Published:2000-11-10
Contact: ZHOU Yong-Hong

摘要/Abstract

摘要：

利用随机扩增多态性DNA(RAPD)技术分析了14种仲彬草属Kengyilia植物的种间关系。对34个OPRON公司十聚体随机引物进行多态性筛选，20个(58．8％)能产生多态性。14个引物产生的112个DNA片断，用于计算种间Jaccard遗传相似性系数分析，在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明：(1)14个Kengyilia物种存在较大的遗传多样性；(2)青藏高原的物种与新疆的物种的RAPD变异极大；(3)形态相似、地理分布一致的物种有一定的亲缘关系，聚类在一起；(4)RAPD结果与形态学和细胞学等分析结果一致。RAPD分析方法将为Kengyilia系统分类提供DNA水平上丰富的资料。

关键词: 仲彬草属, RAPD分析, 遗传变异, 亲缘关系, 形态学

Abstract:

To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were

used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic

products. A total of 112 products amplified from 14 primers were selected for RAPD analysis.

Eighty-three (74.1%) amplified products were found to be polymorphic among 14 species. Data

were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA

in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic

diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the spe-

cies from the Qinghai-Xizang Plateau and those from Xinjiang; (3) The species with similar morpho-

logical characters and the species from the same areas or neighboring geographical regions were clus-

tered together; (4) RAPD results are comparable with those obtained from studies on morphology

and cytology. RAPD assay is a useful additional method for assessing the relationships among the

species in Kengyilia.

Key words: Kengyilia, RAPD assay, Genetic variation, Relationships, Morphology

周永红,郑有良,杨俊良,颜济,贾继增. 利用RAPD分子标记评价仲彬草属的种间关系[J]. 中国科学院大学学报, 2000, 38(6): 515-521.

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YEN Chi, JIA Ji-Zeng. Relationships among Kengyilia species assessed by RAPD markers[J]. , 2000, 38(6): 515-521.

[1]	尉驰俊, 邱越, 袁丁, 郑庭辉. 腹主动脉瘤形态学二维断面和三维立体测量的误差分析[J]. 中国科学院大学学报, 2018, 35(5): 607-611.
[2]	周志钦, 潘开玉, 洪德元. 芍药属牡丹组基于形态学证据的系统发育关系分析[J]. 中国科学院大学学报, 2003, 41(5): 436-446.
[3]	陈劲枫, 庄飞云, 逯明辉, 钱春桃, 任刚. 采用SSR和RAPD标记研究黄瓜属（葫芦科）的系统发育关系[J]. 中国科学院大学学报, 2003, 41(5): 427-435.
[4]	张海琴,周永红,郑有良,杨瑞武,丁春邦. 长芒猬草与华山新麦草属间杂种的形态学和细胞学研究[J]. 中国科学院大学学报, 2002, 40(5): 421-427.
[5]	张树仁. 囊状嵩草(莎草科)及相关类群的分类[J]. 中国科学院大学学报, 2002, 40(5): 433-443.