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中国科学院大学学报 ›› 2002, Vol. 40 ›› Issue (5): 421-427.DOI: 10.7523/j.issn.2095-6134.2002.5.005

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长芒猬草与华山新麦草属间杂种的形态学和细胞学研究

张海琴,周永红,郑有良,杨瑞武,丁春邦   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2002-09-10
  • 通讯作者: 张海琴

Morphology and cytology of intergeneric hybrids between Hystrix duthiei ssp. longearistata and Psathyrostachys huashanica (Poaceae: Triticeae)

ZHANG Hai-Qin, ZHOU Yong-Hong, ZHENG You-Liang, YANG Rui-Wu, DING Chun-Bang   

  • Received:1900-01-01 Revised:1900-01-01 Published:2002-09-10
  • Contact: ZHANG Hai-Qin

摘要:

为研究长芒猬草Hystrix duthiei ssp.longearistata的染色体组成,将其与华山新麦草Psathyrostachys
huashanica进行了人工杂交,获得杂种F1。对亲本及杂种F1,花粉母细胞减数分裂染色体配对行为、繁育
特性和形态特征进行了比较分析。结果表明:杂种F1的许多形态特征介于父母本之间,花粉完全不育,
结实率为0;杂种F1花粉母细胞减数分裂中期Ⅰ染色体配对较高,55.12%的细胞形成5个或6个二价
体,其构型为:9.83Ⅰ+5.46Ⅱ+0.07Ⅲ,C-值为0.57。以上结果表明H.duthiei ssp.Longearistata含有
Ns染色体组。本文还讨论了Hystrix与Leymus的关系。

关键词: 长芒猬草, 华山新麦草, 属间杂交, 减数分裂, 染色体组, 染色体配体, 亲缘关系

Abstract:

 In order to determine its genomic constitution,  Hystrix duthiei ssp.  longearistata was
crossed with Psathyrostachys huashanica. Hybrids were obtained successfully from this combination.
Morphology, fertility and chromosome pairing behavior in meiosis of the parents and their hybrids F1
were investigated. The results showed obvious morphological differences between H. duthiei ssp.
longearistata and P. huashanica, and intermediate spikes of the F1 plants between the two parental
species. The hybrids were completely sterile. Chromosome pairing at MI of PMCs in F1 hybrids was
relatively high and 55.12% of PMCs showed 5 or 6 bivalents per cell. Meiotic configurations were
9.83 Ⅰ + 5.46 Ⅱ+ 0.07 Ⅲ, with C-value of 0. 57, which indicated that H. duthiei ssp.
longearistata and P. huashanica should share one Ns genome. The relationship between Hystrix
and Leymus was also discussed.

Key words: Hystrix duthiei ssp. Longearistata, Psathyrostachy huashanica, Intergeneric hybrid, Meiosis, Genome, Chromosome pairing, Relatipnship