11种金花茶植物的RAPD分析及其系统学意义

中国科学院大学学报

11种金花茶植物的RAPD分析及其系统学意义

施苏华,唐绍清,陈月琴,屈良鹄,张宏达

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-10 发布日期:1998-07-10
通讯作者: 施苏华

Phylogenetic Relationships Among Eleven Yellow-Flowered Camellia Species Based on Random Amplified Polymorphic DNA

SHI Su-Hua, TANG Shao-Qing, CHEN Yue-Qin, QU Liang-Hu, CHANG Hung-Ta

Received:1900-01-01 Revised:1900-01-01 Online:1998-07-10 Published:1998-07-10
Contact: SHI Su-Hua

摘要/Abstract

摘要：

利用随机扩增多态性DNA(RAPD)技术分析了11种金花茶植物，即:龙州金花茶Camellia

longzhouensis J.Y．Luo，弄岗金花茶C．longgangensis C.F.Liang et S．L. Mo，大样金花茶 C．grandis(C.

F. Liang et S．L．Mo)H．T，Chang et S．Y.Liang，扶绥金花茶 C.fusuiensis S．Y. Liang et X．J.Dong，中东金花茶C．achrysantha H．T．Chang et S．Y．Liang，小花金花茶C. micrantha S．Y. Liang et Y．C．Zhong，薄叶金花茶C．chrysanthoides H．T．Chang，毛籽金花茶C．ptilosperma S．Y．Liang et Q．D．Chen，陇瑞金花茶C．longruiensis S．Y．Liang et X．J．Dong，凹脉金花茶C．impressinervis H．T．Chang et S．Y．Liang和夏石金花茶C．xiashiensis S. Y．Liang et C．Z. Deng。选用14个引物(10 bp)扩增出201个DNA片段，用于

种间遗传相似性分析，构建系统发育树图。分析结果表明:(1)小花金花茶、扶绥金花茶与中东金花茶之间的关系较近；(2)大样金花茶、薄叶金花茶和夏石金花茶之间的关系较近；(3)龙州金花茶、毛籽金花茶、陇瑞金花茶和弄岗金花茶之间的关系较近；(4)凹脉金花茶与其他各种关系相对较远。

关键词: 金花茶植物, RAPD, 系统发育关系

Abstract:

Eleven species of yellow-flowered Camellia were analyzed using random amplified

polymorphic DNA (RAPD) markers. Fourteen decanucleotide primers of arbitrary sequences

were selected from 140 primers for DNA amplification. 201 bands were used as markers.

Based on the RAPD data, a phenogram has been constructed. The result shows that: (1)

C. micrantha and C. fusuiensis are closely related to C. achrysantha ; (2) C. chrysan-

thoides and C. grandis form a monophyletic group with C. xiashiensis; (3) There is a

close relationship among C. longzhouensis, C. ptilosperma, C. longruiensis, which form

another monophyletic group; (4) C. impressinervis is highly divergent from all the other

taxa.

Key words: Yellow-flowered Camellia, RAPD, Phylogenetic relationship

施苏华,唐绍清,陈月琴,屈良鹄,张宏达. 11种金花茶植物的RAPD分析及其系统学意义[J]. 中国科学院大学学报.

SHI Su-Hua, TANG Shao-Qing, CHEN Yue-Qin, QU Liang-Hu, CHANG Hung-Ta. Phylogenetic Relationships Among Eleven Yellow-Flowered Camellia Species Based on Random Amplified Polymorphic DNA[J]. .

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