中国红豆杉属及其近缘植物的遗传变异和亲缘关系分析

中国科学院大学学报

中国红豆杉属及其近缘植物的遗传变异和亲缘关系分析

周其兴,葛颂,顾志建,岳中枢

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-10 发布日期:1998-07-10
通讯作者: 周其兴

Genetic Variation and Relationships Within Taxus and Between the Genus and Pseudotaxus in China

ZHOU Qi-Xing, GE Song, GU Zhi-Jian, YUE Zhong-Shu

Received:1900-01-01 Revised:1900-01-01 Online:1998-07-10 Published:1998-07-10
Contact: ZHOU Qi-Xing

摘要/Abstract

摘要：

采用水平式淀粉凝胶电泳技术，对中国产红豆杉属Taxus 3种1变种以及白豆杉Pseudotaxus

chienii共12个居群的遗传多样性进行了检测。对7个酶系统9个酶位点的分析结果表明，红豆杉属和

白豆杉的种内遗传多样性水平较高，各类群的多态位点比率(P)为44.4％～77.8％，等位基因平均数

(A)为1．6～2．1，平均期望杂合度(He)和实际观察杂合度(Ho)分别为0.065～0.152和0.068～0.111。

根据对等位酶实验结果的分析，结果表明除云南红豆杉T．yunnanensis与红豆杉属其它几个种的遗传

一致度稍低(I=0.727～0.804)外，红豆杉属的其它几个种的遗传一致度较高(0.906～0.995)，体现其

亲缘关系很近。笔者建议将红豆杉属的这几个种合并为一个种，并根据地理分布及不同生境发生的变

异在种下设地理宗。同时根据等位酶的实验分析结果，白豆杉和红豆杉属各种的比较有显著差异，支持

白豆杉作为单独属Pseudotaxus的处理。

关键词: 红豆杉属, 白豆杉属, 遗传变异, 种间关系

Abstract:

In the present paper, genetic diversity of 12 populations of four Taxus taxa and

one species of monotypic genus Pseudotaxus in China were studied by horizontal starch gel

electrophoresis. Seven enzymes encoded by nine loci were assessed. Genetic diversity within

5 taxa were relatively high with the proportion of polymorphic loci ( P ) ranging from 44.4 %

to 77.8 %, average number of alleles per locus (A) from 1.6 to 2.1; mean expected and ob-

served heterozygosity ( He, Ho) per locus from 0. 065 to 0.152 and 0. 068 to 0.111, respec-

tively. Combined with the analysis of other characters, the results from allozyme analysis

suggest that there be very close relationships between the three species and one variety in

Taxus, with high genetic identities (I) between them(from 0. 727 to 0. 995) except those

between T. yunnanensis and other taxa in Taxus (0. 727～ 0. 804). Consequently, we pro-

pose that these taxa in Taxus be treated as different geographical races within a single

species. Also the taxonomic position of Pseudotaxus as a distinct genus is supported by our

allozyme data.

Key words: Taxus, Pseudotaxus, Genetic variation, Interspecific relationship

周其兴,葛颂,顾志建,岳中枢. 中国红豆杉属及其近缘植物的遗传变异和亲缘关系分析[J]. 中国科学院大学学报.

ZHOU Qi-Xing, GE Song, GU Zhi-Jian, YUE Zhong-Shu. Genetic Variation and Relationships Within Taxus and Between the Genus and Pseudotaxus in China[J]. .

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[2]	周永红,郑有良,杨俊良,颜济,贾继增. 利用RAPD分子标记评价仲彬草属的种间关系[J]. 中国科学院大学学报, 2000, 38(6): 515-521.
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