苔藓植物光合作用荧光光谱和动力学荧光的比较

中国科学院大学学报

苔藓植物光合作用荧光光谱和动力学荧光的比较

施定基, 吴鹏程, 仇嫒嫒, 汪楣芝

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1992-08-10 发布日期:1992-08-10
通讯作者: 施定基

Comparative Studies on Photosynthetic Fluorescence Spectra and Fluorescence Kinetics of Bryophytes

Shi Ding-Ji, Wu Pan-Cheng, Qiu Yuan-Yuan, Wang Mei-Zhi

Received:1900-01-01 Revised:1900-01-01 Online:1992-08-10 Published:1992-08-10
Contact: Shi Ding-Ji

摘要/Abstract

摘要：

本文首次比较了光合作用的荧光光谱和荧光动力学在苔藓植物的原始种类和进化种类之间的异同。原始的和进化的苔藓植物具有发射波长相同的室温荧光光谱，其发射高峰位于686-690nm(来自光系统Ⅱ)和736-740nm(来自光系统Ⅰ)。而它们的低温(77K)荧光光谱有三个发射峰：F_687-689和F_697-699来自光系统Ⅱ，F_723-734来自光系统I。前两个峰在原始的和进化的种类中基本相同。按第三个发射峰可把被测的苔藓植物分为两组：发射峰在725nm左右的有细牛毛藓、长肋对齿藓、对齿藓、斜叶芦荟藓、密集匍灯藓和地钱，它们是较原始的藓类和较进化的苔类；发射峰在732nm左右的有细枝羽藓、东亚金灰藓、鼠尾藓、鳞叶藓、粗枝藓和美灰藓等较进化的藓类，也有较原始的钝叶匍灯藓。已知光系统I核心复合物CPI的77K荧光发射峰在722nm，而CPIa(核心复合物与外周天线复合物)和LHC-I(外周天线复合物)的发射峰在730nm。这说明在苔藓植物进化过程中，光系统Ⅱ比较保守；而光系统Ⅰ有所变化，原始的藓类主要含有光系统Ⅰ核心复合物，而较进化的藓类才含有较完善的外周天线复合物。光合作用荧光动力学分析表明，在原始藓类和地钱中具有较低的光系统Ⅱ活性、光系统Ⅱ的原初光能转换效率、光合碳同化和潜在的光合量子转换效率；而较进化的具有较高的活性和效率。但是，原始的密集匍灯藓也具有较高的活性和效率，而进化的美灰藓却具有较低的活性和效率。这可能表明这两种植物是由原始向进化发展过程中的中间类型。

关键词: 苔藓植物, 荧光发射光谱, 荧光动力学, 光合作用进化, 光系统I的核心复合物和外周天线色素复合物, 光系统II

Abstract:

Bryophytes are the transitional forms from water habitants to

terrestrials, however, there have been only a few works on their photosynthesis.

It was the first time to study on photosynthetic fluorescence spectra and

fluorescence kinetics of primitive and advanced species comparatively. Both the

primitive and advanced ones had the same fluorescence spectra at room

temperature, which contained two maximum emissions: F_686-690 from the

Photosystem II and F_736-740 from the Photosystem I. And then, there were three

maximum emissions in the fluorescence spectra at 77K :F_687-689 and F_697-699 from

Photosystem II, and F_723-734 from Photosystem I. The first two maximum

emissions were the same for both the primitive and advanced species. According

to the third maximum emission the bryophytes under study fell into two

categories: The first one possessing the maximum emission around 725 nm,

including Ditrichum flexicaule , Didymodon icmadophyllum , Didymodon

rigidicaulis, Aloina obliquifolia, Plagiomnium confertidens and Marchantia

polymorpha, which were primitive mosses and advanced liverwort. The second

one possessing the maximum emission around 732nm, including Thuidium

delicatulum , Pylaisia brotheri , Myuroclada maximowiczii , Taxiphyllum

taxirameum, Gollania neckerella, Eurohypnum leptothallum, which were advanced

mosses, and the primitive one Plagiomnium rostratum. The characteristics of

fluorescence spectra implied that the Photosystem II was conservative and

Photosystem I was changeable during bryophyte evolution. The primitive mosses

possess mainly the PSI core complex (CPI) and then the advanced species contain

both CPI and LHC-I. In analysis of photosynthetic fluorescence kinetics,

Fv/(Fc+Fv) is a measure of the activity of the Photosystem II; Fv/Fm is dependent

on efficiency of primary photoconversion in the Photosystem II; Fm/(Fo+Fv) is

related to photosynthetic carbon assimilation; and Fd/Fs is a measure of the

potential photosynthetic quantum conversion. The fluorescence kinetics of the

bryophyte photosynthesis showed that the Photosystem II activity, the efficieiency of

primary photoconversion in Photosystem II, the photosynthetic carbon assimila-

tion and the efficiency of the potential photosynthetic quantum conversion in

primitive species, such as Ditrichum flexicaule, Didymodon icmadophyllus, D.

rigidicaulis, Plagiomnium rostratum and the liverwort Marchantia polymorpha,

were lower than those in the advanced species, Myuroclada maximowiczii,

Pylaisia brotheri , Gollania neckerella Taxiphyllum taxirameum , Thuidium

delicatulum. However, the primitive Plagiomnium confertidens was of the high

activities and efficiencies and the advanced Eurohypnum leptothallum was of low

ones. It seemed that P. confertidens and E. leptothallum were an intermediate

from the primitive to the advanced.

Key words: Bryophytes, Photosynthetic fluorescence spectrum, Fluorescence kinetics, Photosynthesis evolution, Photosystem I Core complex and antenna complex, Photosystem II

施定基, 吴鹏程, 仇嫒嫒, 汪楣芝. 苔藓植物光合作用荧光光谱和动力学荧光的比较[J]. 中国科学院大学学报.

Shi Ding-Ji, Wu Pan-Cheng, Qiu Yuan-Yuan, Wang Mei-Zhi. Comparative Studies on Photosynthetic Fluorescence Spectra and Fluorescence Kinetics of Bryophytes[J]. .

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